Abstract: The Δ⁶-fatty acid desaturase gene from Oenothera bienniswas introduced into the explants of Brassica napus in this study，and postive transgenic plantlets were confirmed with PCR which contribute to provide the primary basis for the future application of gene engineering to γ-linolenic production． This study used cotylendon node explant to optimize Agrobacterium-mediated Brassica napusgenetic transformation system． The optimal pre-culture condition for the cotylendon node is two days on MS medium + 6-BA 2． 0 mg /L + NAA 0． 2 mg /L． Agrobacterium was transformed with binary vector pPZP221 harboring 35S promoter-driven ObD6D gene，which was used to infect Brassica napuscotylendon node with optimal concentration OD600 = 0． 4 and time 5 min． Infected explants were cultured on differential selective medium with gentamicin and the resisitant explants were subsequently transfered to root-promoting medium． Postive transgenic plantlets were detected by PCR and transfered to soil． In total，we got twelve transgenic plantets．

Key words: Brassica napus, Oenothera biennis, Δ⁶-fatty acid desaturase gene, γ-linolenic acid, Agrobacteriummediated

CLC Number: 

• S565． 4