Immunoscreening and Sequencely Organization of the Helicoverpa armigera cDNA Expression Library for cDNA Clones

doi:10.7668/hbnxb.2010.02.004

ACTA AGRICULTURAE BOREALI-SINICA ›› 2010, Vol. 25 ›› Issue (2): 18-22. doi: 10.7668/hbnxb.2010.02.004

Special Issue: Cotton；

Previous Articles Next Articles

Immunoscreening and Sequencely Organization of the Helicoverpa armigera cDNA Expression Library for cDNA Clones

LI Jie¹, ZHANG Xia², GUO Wei³, LIU Xiao-min¹, LI Xin-na¹, ZHAO Dan⁴

1. College of Plant Protection, Agricultural University of Hebei, Biological Control Centre of Plant Diseases and Pests of Hebei Province, Baoding 071000, China;
2. College of Life Science, Agricultural University of Hebei, Baoding 071000, China;
3. Biological Institute of Hebei, Shijiazhuang 050051, China

Received:2009-12-27 Published:2010-04-28

Abstract

Abstract: The peritrophic matrix(PM)is a physical barrier protecting the midgut.Destruction of peritrophic membrane can make the midgut in an abnormal physiological state,eventually leading to death.Helicoverpa armigera(Hübner)larval midgut cDNA expression library was immunoscreened with an anti-PM proteins polyclonal antiserum which has been successfully prepared by immuning a rabbit,385 positive clones were obtained from several screening.26 purified cDNA clones of them were chosen for sequencing.The results showed that 19 cDNAs of the above 26 cDNA clones encoded insect intestinal mucin(IIM).The PM protein composition was then analyzed,and the result showed that there were a lot of glycopreteins by PAS staining analysis.By isolating and identifying the PM proteins of Helicoverpa armigera,we could get a clear idea of the physiological function of PM protein.We could also hunt for novel target site in insect pest management and explore high efficient biological pesticide.

Key words: Helicoverpa armigera, cDNA expression library, Immunoscreening, Mucin, PAS staining

CLC Number:

Q785

LI Jie, ZHANG Xia, GUO Wei, LIU Xiao-min, LI Xin-na, ZHAO Dan. Immunoscreening and Sequencely Organization of the Helicoverpa armigera cDNA Expression Library for cDNA Clones[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 18-22. doi: 10.7668/hbnxb.2010.02.004.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2010.02.004

http://www.hbnxb.net/EN/Y2010/V25/I2/18

References

[1] Balbiani E G.Etudes anatomiques et histologiques sur le tube digestif des Crytops[J].Arch Zool Exp Gen,1890,8:81-82.
[2] Lehane M J.Peritrophic matrix structure and function[J].Annu Rev Entomol,1997,42:525-550.
[3] Peters W.Peritrophic membranes[M].Springer,New York,1992.
[4] Tellam R L,Wijffels G,Willadsen P.Peritrophic matrix proteins[J].Insect Biochem Mol Biol,1999,29(2):87-101.
[5] Shi X,Chamankhah M,Visal-Shah S,et al.Modeling the structure of the type I peritrophic matrix:characterization of a Mamestra configurata intestinal mucin and a novel peritrophin containing 19 chitin binding domains[J].Insect Biochem Mol Biol,2004,34(10):1101-1115.
[6] Wang P,Granados R R.Calcofluor disrupts the midgut defense system in insects[J].Insect Biochem Mol Biol,2000,30(2):135-143.
[7] Zhu R,Liu K,Peng J,et al.Optical brightener m²R destroys the peritrophic membrane of Spodoptera exigua(Lepidoptera:Noctuidae)larvae[J].Pest Manag Sci,2007,63(3):296-300.
[8] 曹佐武.糖蛋白PAGE分离后的糖基显色法[J].生物技术通报,2006,5:87-89.
[9] 张倩,杨振,张艳贞,等.蛋白质糖基化修饰的研究方法及其应用[J].生物技术通报,2006,1:46-49.
[10] 张霞,李国勋,郭巍.甜菜夜蛾粘蛋白cDNA基因的分子克隆与序列分析[J].中国农业科学,2008,42(11):3898-3904.
[11] 刘可人,金美芳,吴士良.O-糖基化位点预测及糖基化酶催化特点[J].生命的化学,2006,26(1):25-27.
[12] Wang P,Granados R R,Molecular cloning and sequencing of a novel invertebrate intestinal mucin cDNA[J].J Biol Chem,1997.272(26):16663-16669.
[13] Shi X,Chamankhah M,Visal-Shan S,et al.Modeling the structure of the type I peritrophic matrix:Characterization of a Mamestra configurata intestinal mucin and a novel peritrophin containing 19 chitin binding domains[J].Insect Biochem Mol Biol,2004,34:1101-1115.

Please choose a citation manager

Content to export

Immunoscreening and Sequencely Organization of the Helicoverpa armigera cDNA Expression Library for cDNA Clones

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	LI WenGUO Wei, ZHANG Xia. Immune-screening and Sequence Analysis of the Midgut cDNA Expression Library from Spodoptera exigua [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 33-37.
[2]	WANG Rong-yan, WANG Jin-yao, CAO Wei-ping, DU Li-xin, SONG Jian, FENG Shu-liang. The Occurrence of Microsporidiosis (Nosema sp.) Infecting Helicoverpa armigera in Field and Its Pathogenicity [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 248-251.
[3]	GUO Jian-ying, WAN Fang-hao, REN Cheng-cai, HAN Zhao-jun. Enhanced Pest Control Efficacy in Transgenic Insect-resistant Cotton Field by Mass-release of Trichogramma chilonis [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 197-202.
[4]	SHI Cui-hong, SONG Ping, WANG Qin-ying, YANG Jun, CUI Long, KONG Fan-fang. *Effects of the Protein Toxin from Xenorhabdus nematophila* on Several Enzymatic Activities of the Helicoverpa armigera Larvae** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(3): 105-110.

模态框（Modal）标题

Please choose a citation manager

Content to export

Immunoscreening and Sequencely Organization of the Helicoverpa armigera cDNA Expression Library for cDNA Clones

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments