Abstract: CTAB and TRIzol reagent methods and RNAplant kit method were chosen for a comparison analysis of to??tal RNA extraction from Myrmecia incisa. Total RNA extracted by CTAB method had the highest yield, but the worst qual??ity due to possible contamination of proteins or polysaccharides.The purity of total extracted RNA by TRIzol method wasslightly higher than that by CTAB, but the lowest yield( 52 g/ 100mg fresh weight of algae), as well the occurrence of anunexpected DNA band revealed by electro phoresis. By use of the RNAplant kit method, the ratio value of OD260 nm/OD280 nm of total extracted RNA was about 2.0, as well its clear electrophoresis pattern showing the good quality and highpurity. The double strand cDNAs synthesized by reverse transcription with the total RNA extracted by RNAplant kitmethod ranged from 500 bp to 2 kb in size, and a specific fragment of M. incisa 18 S rRNA gene was amplified by RT??PCR with the synthesized cDNA as a template.These results demonstrated that the RNAplant kit method could be suitable for total RNA extraction from this alga and could meet the needs of most molecular biological experiments including gene cloning and expression analysis.

Key words: Myrmecia incisa, RNA extract ion, RT??PCR

CLC Number: 

• Q813