ACTA AGRICULTURAE BOREALI-SINICA ›› 2008, Vol. 23 ›› Issue (6): 101-104. doi: 10.7668/hbnxb.2008.06.023

Special Issue: Cucumber Plant protection

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Construction of Engineered Strains of Pseudomonas fluorescence and Its Control Effects on Cucumber Fusarium Wilt Disease

YUE Dong-xia, ZHANG Yao-wu, CHEN Rong, XU Chang-ai   

  1. Tianjin Research Centre for Agricultural Biotechnology, Tianjin 300192, China
  • Received:2007-12-21 Published:2008-12-28

Abstract: The plasmid pMON5122 containing 2,4-DAPG synthesis gene had been introduced into wild strains ofP.f luorescenceby engineering approach.The effects of different heat shock hours,different concentration of CaCl2and different growth phase ofP.fluorescenceon the transformation frequency were tested,and the control effect on cucumber fusarium wilt disease by engineered strains ofP.f luorescencewas invest igated.The results showed that it was beneficial to the transformation ofP.fluorescencewhen OD600was about 0.53 and the cells were treated with 0.025 mol/L CaCl2and heated for 3-4 min at 42.The results from pot experiment test showed that the control effect of the engineered strains on the cucumber wilt disease was 75.0% and the rate of emergence of cucumber seeds was raised.

Key words: Pseudomonas fluorescence, Engineered strains, Cucumber fusarium wilt disease, Metabolic product, Bio- logical pesticide

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Cite this article

YUE Dong-xia, ZHANG Yao-wu, CHEN Rong, XU Chang-ai. Construction of Engineered Strains of Pseudomonas fluorescence and Its Control Effects on Cucumber Fusarium Wilt Disease[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 101-104. doi: 10.7668/hbnxb.2008.06.023.

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