Abstract:
Tetraploids were induced from in vitro Aloe vera L by soaking shoot segments in colchicine solution and adding colchicine in the culture medium.The results showed that the adding colchicine in the culture medium increased the efficiency of induced tetraploids than one of soaking treatment.The optimal conditions for inducing tetraploids were adding colchicine in the culture medium for 3 to 4 d with colchicine at a concentration of 2 000 mg/L.The rate of induction was 41.5%.Compared with the diploids,the appearance of variations is distinct including plumper leaves and more chloroplasts in stomatal guard cells.
Key words:
Aloe vera L,
Colchicines,
Multiploid
CLC Number:
REN Qing, LI Shou-mian, YAO Bin, WANG Xiao-xiao. Studies on Induction of Autotetraploid of Aloe vera L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(S2): 136-138. doi: 10.7668/hbnxb.2007.S2.034.