Somatic Embryo Induction and Cloning from Hypocotyledonary in Onobrychis Viciaefolia Scop

doi:10.3321/j.issn:1000-7091.1989.03.003

Abstract

Abstract: Hypocotylar explants of Onobrychis viciaefolia Scop were cultured on LS basal medium-supplemented with 1 mg/l BA, 1mg/l KT. Two weeks after of culture calli were initiated on the surface of sections. Light-yellow callus from one explants was selected and proliferated on LS medium. And then it transferrated to somatic embryo induction medium(LS basal medium with lmg/1 BA). Contact embryogenic calli containing a large number of globular embryos were found after 15 days of embryogenic culture, but only a few globular embryos can develop normally. Another new possible pathway for in vitro development is. buds and roots were formed on the surface of globular-like structure and it is different from normal organogenesis because of its individual structure.

Key words: Sainfoin, Tissue culture, Somatic embryo, Cloning

Lu Tiegang, Zheng Guochang. Somatic Embryo Induction and Cloning from Hypocotyledonary in Onobrychis Viciaefolia Scop[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1989, 4(3): 11-15. doi: 10.3321/j.issn:1000-7091.1989.03.003.

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