Abstract: A comparative electrophoretic analysis of gliadins and glutenins for 18 native Chinese cultivars extracted with different methods was made by using different gradient SDS polyacrylamide gel.The results reported here indicated that after removing most of gliadins,albumins and other alcohol-soluble substances by using 1M urea,those HMW(high molecular weight)glutenins and gliadins extracted with lysis buffer containing 2% SDS and 5% ?-mercaptoethanol showed a high resolution and considerable polymorphism in an 8-12% gradient polyacrylamide SDS gel.It is considered,therefore,this procedure may be useful in the cultivar identification of wheat.Besides,the total proteins extracted directly from half seed with lysis buffer may be useful for single seed characterization of wheat cultivars.

Key words: electrophoretic analysis, wheat cultivar identification