华北农学报 ›› 2020, Vol. 35 ›› Issue (2): 187-195. doi: 10.7668/hbnxb.20190507

所属专题: 水稻 生物技术

• 资源环境·植物保护 • 上一篇    下一篇

NaCl对不同抗性水稻氮代谢及相关基因表达的影响

杨德翠1, 徐青1, 赵方贵1, 柳冕2, 刘新1   

  1. 1. 青岛农业大学 生命科学学院, 山东省高校植物生物技术重点实验室, 山东 青岛 266109;
    2. 武汉海稻国际生物科技有限公司, 湖北 武汉 430000
  • 收稿日期:2019-10-11 出版日期:2020-04-28
  • 通讯作者: 刘新(1966-),女,山东莱阳人,教授,博士,硕士生导师,主要从事植物营养生理与分子生物学研究。
  • 作者简介:杨德翠(1974-),女,山东菏泽人,讲师,博士,主要从事植物逆境生理及光合生理生态研究。
  • 基金资助:
    山东省重大创新工程项目(2017CXGC0312);武汉海稻国际生物科技有限公司(HDNJY-2016-01);山东省高等学校科技计划项目(J17KA130)

Effects of NaCl Stress on Nitrogen Metabolism and Related Gene Expression of Rice with Different Resistance

YANG Decui1, XU Qing1, ZHAO Fanggui1, LIU Mian2, LIU Xin1   

  1. 1. College of Life Science, Qingdao Agricultural University, Key Lab of Plant Biotechnology in University of Shandong Province, Qingdao 266109, China;
    2. Wuhan Ocean Rice International Biotech Co, Ltd, Wuhan 430000, China
  • Received:2019-10-11 Published:2020-04-28

摘要: 为了探究盐胁迫下不同抗性水稻氮代谢的变化,以抗性品种东稻4号、盐敏感品种日本晴为材料,测定NaCl胁迫下水稻根和叶中氮含量、氮代谢相关酶活及相关基因表达的变化。结果表明,0.3%的NaCl胁迫后水稻根和叶中NO3-含量、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、依赖NADH的谷氨酸合酶(NADH-GOGAT)活性均有不同程度的下降,而NH4+含量、依赖Fd的谷氨酸合酶(Fd-GOGAT)、谷氨酸脱氢酶(GDH)、天冬氨酸转氨酶(AST)活性均升高,东稻4号氮含量及氮代谢酶活性比日本晴稳定,根和叶中变化一致。检测氮转运及代谢相关基因表达发现,硝酸根转运蛋白基因OsNRT1;1、OsNRT1;5、OsNRT1;8、OsNRT2;1,铵转运蛋白基因OsAMT1;1、OsAMT1;2及谷氨酰胺合成酶基因OsGS1;2、天冬氨酸转氨酶基因OsAS盐胁迫后在2个品种中均升高,在高表达部位升高显著;谷氨酸合酶基因OsFd-GOGAT在两品种叶中升高显著,根中稍有下降;谷氨酸合酶基因OsNADH-GOGAT和谷氨酸脱氢酶基因OsGDH在日本晴根中升高显著,在叶中及东稻4号中升高但不显著。OsNR1在NaCl胁迫后两品种叶中表达量显著降低,根中无差异。东稻4号OsNRTs及氮代谢相关基因表达量总体上高于日本晴,而日本晴OsAMTs升高幅度大于东稻4号。研究表明,NaCl胁迫下,东稻4号相比日本晴更能保持氮代谢稳定性,这可能是水稻抗性品种耐盐的一个重要机制。

关键词: 水稻, 盐胁迫, 氮代谢, 基因表达

Abstract: In order to study the changes of nitrogen metabolism of rice varieties with different resistant under salt stress, the resistant rice Dongdao 4 and the sensitive variety Nipponbare were used as materials, and changes of nitrogen content, nitrogen-related enzymes and gene expression in rice roots and leaves under NaCl stress were investigated. The results showed that the nitrate nitrogen content, nitric acid reductase (NR) activity and glutamine synthetase(GS), NADH-dependent glutamate synthase (NADH-GOGAT) enzyme activity of rice seedings treated by 0.3% NaCl in roots and leaves reduced. While the ammonium nitrogen content, the activity of ferrodoxin-dependent glutamate synthase (Fd-GOGAT), glutamate dehydrogenase(GDH) and aspartic acid aminotransferase(AST) increased. The nitrogen content and nitrogen metabolic enzyme activity of Dongdao 4 were more stable than Nipponbare, and the changes of roots and leaves were consistent. Detection of genes expression associated with nitrogen transport and metabolism found that: The expression of nitrate transporter protein genes OsNRT1; 1, OsNRT1; 5, OsNTR1; 8, OsNRT2; 1, ammonium transporter protein genes OsAMT1; 1, OsAMT1; 2, glutamine synthase gene OsGS1; 2, transaminase gene OsAS had high expression after NaCl stress and significantly increased in the high expression tissue. Glutamate synthase gene OsFd-GOGAT increased significantly in the leaves of both cultivars and decreased slightly in the roots. Gutamate synthase gene OsNADH-GOGAT and glutamate dehydrogenase gene OsGDH were elevated significantly in the roots of Nipponbare, but not significantly in leaves and also in the Dongdao 4. The expression of OsNR1 was significantly reduced in leaves of the two varieties, but no difference in roots.On the whole the expression of OsNRTs and the genes related to nitrogen metabolism in Dongdao 4 were higher than that of Nipponbare, but OsAMTs in Nipponbare increase more than Dongdao 4. The study showed that Dongdao 4 could maintain more stable nitrogen metabolic than Nipponbare, and this might be an important mechanism for the salt tolerance of rice resistant variety.

Key words: Rice, Salt stress, Nitrogen metabolism, Gene expression

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引用本文

杨德翠, 徐青, 赵方贵, 柳冕, 刘新. NaCl对不同抗性水稻氮代谢及相关基因表达的影响[J]. 华北农学报, 2020, 35(2): 187-195. doi: 10.7668/hbnxb.20190507.

YANG Decui, XU Qing, ZHAO Fanggui, LIU Mian, LIU Xin. Effects of NaCl Stress on Nitrogen Metabolism and Related Gene Expression of Rice with Different Resistance[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 187-195. doi: 10.7668/hbnxb.20190507.

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