Study on Transformation in Maize of Pollen Lethal Gene ZmAA1
MENG Lingcong1, SONG Guangshu1,2, LÜ Qingxue1, LIU Hongwei1,2, ZHANG Zhijun1,2, LI Chunlei1, WANG Min1, LIU Wenguo1,2
1. Jilin Jinong Agricultural New and Hi-Tech Develop Co., Ltd, Gongzhuling 136100, China;
2. Jilin Academy of Agricultural Sciences, Changchun 130124, China
Abstract：In order to study the function of pollen lethal gene ZmAA1 and create tansgenic male sterile materials. ZmAA1 and its specific promoter Pg47 were found according to GenBank,designed to plus restriction endonuclease on the downstream and inserted them into an cloning vector puc57,construction of vector pCAMBIA3300-Pg47-ZmAA1-35S-bar used traditional construction methods digested connection.A maize inbred line Zheng 58 was infected by Agrobacterium tumefacfaciens with the vector pCAMBIA3300-Pg47-ZmAA1-35S-bar, 94 PPT-resistant seedlings were obtained and 47 plants were PCR positive.The pollen sterility in greenhouse RT-PCR assay for gene bar in the transgenic plant leaves and immuno strip test suggested that pollen lethal gene ZmAA1 had been integrated into the maize genome,and protein was expressed.
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2016, Vol. 31 
