湖羊<i>CEL</i>基因多态性及其与眼肌面积性状的关联分析

doi:10.7668/hbnxb.20195076

华北农学报 ›› 2024, Vol. 39 ›› Issue (5): 231-238. doi: 10.7668/hbnxb.20195076

所属专题： 畜牧；

• 畜牧·水产·兽医 • 上一篇

湖羊CEL基因多态性及其与眼肌面积性状的关联分析

蔡有鑫¹, 王维民², 田慧彬², 张德印², 赵源², 张煜坤², 徐丹², 杨晓斌², 马宗武¹, 黄志强¹, 刘晓强¹, 何丽娟¹, 韩坤潮², 吴伟伟³, 高飞⁴, 王立忠⁴, 张小雪¹

¹ 甘肃农业大学动物科学技术学院,甘肃兰州 730070
² 兰州大学草种创新与草地农业生态系统全国重点实验室,甘肃兰州 730000
³ 新疆畜牧科学院,新疆乌鲁木齐 830000
⁴ 金川集团股份有限公司居佳生态农业有限公司,甘肃金昌 737103

收稿日期:2024-04-28 出版日期:2024-10-28
通讯作者:
张小雪(1984-),女,湖北武汉人,副教授,博士,硕士生导师,主要从事动物遗传育种研究。
作者简介:
蔡有鑫(1998-),男,青海门源人,在读硕士,主要从事动物遗传育种研究。
基金资助:
国家重点研发计划(2022YFD1302000); 国家自然科学基金项目(32260818); 金川集团股份有限公司2023年重大科研项目(JKQGJJ23Z202301); 中央引导地方科技发展专项资金项目(ZYYD2022B08)

Analysis of CEL Gene Polymorphisms and Their Association with Eye Muscle Area Traits in Hu Sheep

CAI Youxin¹, WANG Weimin², TIAN Huibin², ZHANG Deyin², ZHAO Yuan², ZHANG Yukun², XU Dan², YANG Xiaobin², MA Zongwu¹, HUANG Zhiqiang¹, LIU Xiaoqiang¹, HE Lijuan¹, HAN Kunchao², WU Weiwei³, GAO Fei⁴, WANG Lizhong⁴, ZHANG Xiaoxue¹

¹ College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China
² State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems,Lanzhou University,Lanzhou 730000,China
³ Xinjiang Academy of Animal Sciences,Urumqi 830000,China
⁴ Jinchuan Group Co.,Ltd.,Jujia,Ecological Agriculture Co.,Ltd.,Jinchang 737103,China

Received:2024-04-28 Published:2024-10-28

摘要/Abstract

摘要：

旨在探讨羧基酯脂肪酶基因(CEL)的单核苷酸多态性与湖羊眼肌面积的相关性。选取1 049只具有准确表型记录且健康的湖羊,屠宰后测定其眼肌面积,采集血液提取基因组DNA,通过PCR和KASPar SNP分型技术对CEL基因进行目的片段扩增和基因分型,并与湖羊眼肌面积进行关联分析,采用RT-qPCR检测CEL基因在湖羊10种组织中的表达情况。结果显示,湖羊CEL基因在不同组织中均有表达,且在十二指肠中表达量最高;该基因存在一个g.4039718 C>T的同义突变,呈中等多态。性状关联分析表明,该多态位点与湖羊的眼肌面积显著关联,TT型个体的眼肌面积显著高于CC型个体。描述性统计结果显示,眼肌面积的变异系数为12.29%,具有较高的选择潜力。相关性分析表明,眼肌面积与体质量、体高、体长、胸围、屠宰率、胴体质量和宰前活质量等生长性状和屠宰性状呈正相关。结果表明,g.4039718 C>T多态位点可作为候选分子标记用于湖羊眼肌面积性状的遗传改良。

关键词: 湖羊, 羧基酯脂肪酶, 眼肌面积, 关联分析

Abstract:

The aim of this study was to investigate the correlation of single nucleotide polymorphisms of the carboxyl ester lipase gene (CEL) with eye muscle area in Hu sheep.The 1 049 Hu sheep with accurate phenotypic records and in good health were selected,their eye muscle area was determined after slaughter,and blood was collected for genomic DNA extraction.Amplification of target fragments and genotyping of CEL gene by PCR and KASPar SNP typing,and association analysis with eye muscle area of Hu sheep,RT-qPCR was used to detect the expression of CEL gene in 10 tissues of Hu sheep.The results showed that the Hu sheep CEL gene was expressed in different tissues and was highest in the duodenum.There was a synonymous mutation of g.4039718 C>T in this gene,which was moderately polymorphic.Trait association analysis showed that this polymorphic locus was significantly associated with eye muscle area in Hu sheep,with TT-type individuals having significantly higher eye muscle area than CC-type individuals.Descriptive statistics showed that the coefficient of variation for eye muscle area was 12.29%,which had high selection potential.Correlation analysis showed that eye muscle area was significantly and positively correlated with growth and slaughter traits such as body weight,body height,breast circumference,slaughter rate,carcass weight and live weight before slaughter.The results indicated that the g.4039718 C>T polymorphic locus could be used as a candidate molecular marker for genetic improvement of eye muscle area traits in Hu sheep.

Key words: Hu sheep, Carboxyl ester lipase (CEL), Eye muscle area, Association analysis

蔡有鑫, 王维民, 田慧彬, 张德印, 赵源, 张煜坤, 徐丹, 杨晓斌, 马宗武, 黄志强, 刘晓强, 何丽娟, 韩坤潮, 吴伟伟, 高飞, 王立忠, 张小雪. 湖羊CEL基因多态性及其与眼肌面积性状的关联分析[J]. 华北农学报, 2024, 39(5): 231-238. doi: 10.7668/hbnxb.20195076.

CAI Youxin, WANG Weimin, TIAN Huibin, ZHANG Deyin, ZHAO Yuan, ZHANG Yukun, XU Dan, YANG Xiaobin, MA Zongwu, HUANG Zhiqiang, LIU Xiaoqiang, HE Lijuan, HAN Kunchao, WU Weiwei, GAO Fei, WANG Lizhong, ZHANG Xiaoxue. Analysis of CEL Gene Polymorphisms and Their Association with Eye Muscle Area Traits in Hu Sheep[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(5): 231-238. doi: 10.7668/hbnxb.20195076.

http://www.hbnxb.net/CN/Y2024/V39/I5/231

图/表 11

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引物功能 Primer functions	引物名称 Primer name	引物序列(5'-3') Primer sequence(5'-3')	产物长度/bp Product length	最佳退火温度/℃ Optimum annealing temperature
PCR扩增引物	CEL-F	CTCGGGACCTGGCCCATCCTCA	360	63
PCR amplification primer	CEL-R	ATTGAACCAGCTCCTCCGGTGA
RT-qPCR 引物	CEL-F	ATGTGCCAGCCATCAACAGCAA	190	61
RT-qPCR primer	CEL-R	ATATCGGTTTCCAGGTCCACCA
	UXT-F	GCAAGTGGATTTGGGCTGTAAC	180	60
	UXT-R	ATGGAGTCCTTGGTGAGGCTGT

引物功能 Primer functions	引物名称 Primer name	引物序列(5'-3') Primer sequence(5'-3')	产物长度/bp Product length	最佳退火温度/℃ Optimum annealing temperature
PCR扩增引物	CEL-F	CTCGGGACCTGGCCCATCCTCA	360	63
PCR amplification primer	CEL-R	ATTGAACCAGCTCCTCCGGTGA
RT-qPCR 引物	CEL-F	ATGTGCCAGCCATCAACAGCAA	190	61
RT-qPCR primer	CEL-R	ATATCGGTTTCCAGGTCCACCA
	UXT-F	GCAAGTGGATTTGGGCTGTAAC	180	60
	UXT-R	ATGGAGTCCTTGGTGAGGCTGT

项目 Items	天数 Days	最小值 Min	最大值 Max	平均值 Average	标准差 s	变异系数/% CV
体质量/kg	100	18.00	23.90	20.73	1.67	8.04
Body weight	140	28.50	34.40	31.55	1.73	5.48
	180	38.50	44.50	41.59	1.73	4.17
体高/cm	100	53.00	58.50	55.48	1.63	2.94
Body height	140	58.50	64.00	61.49	1.65	2.68
	180	62.00	67.00	64.66	1.57	2.43
体长/cm	100	55.50	61.34	58.36	1.66	2.85
Body length	140	63.00	68.50	65.73	1.70	2.59
	180	68.00	73.50	70.69	1.61	2.28
胸围/cm	100	59.50	65.00	62.25	1.77	2.84
Chest circumference	140	68.50	74.00	71.49	1.69	2.37
	180	78.00	83.00	80.50	1.72	2.13
初生质量/kg Birth weight	1	2.60	6.80	4.05	0.76	18.80
断奶质量/kg Weaning weight	56	11.80	17.70	14.49	1.64	11.31
饲料转化率/%	100~140	4.08	9.44	5.70	0.87	15.30
Feed conversion rate	140~180	3.41	9.11	6.96	1.08	15.57
胴体质量/kg Carcass weight	180	20.80	26.70	23.63	1.73	7.34
宰前活质量/kg Live weight before slaughter	180	41.20	47.10	43.83	1.71	3.91
屠宰率/% Dressing percentage	180	0.50	0.56	0.53	0.02	3.08
眼肌面积/cm² Eye muscle area	180	8.95	14.92	11.86	1.46	12.29

项目 Items	天数 Days	最小值 Min	最大值 Max	平均值 Average	标准差 s	变异系数/% CV
体质量/kg	100	18.00	23.90	20.73	1.67	8.04
Body weight	140	28.50	34.40	31.55	1.73	5.48
	180	38.50	44.50	41.59	1.73	4.17
体高/cm	100	53.00	58.50	55.48	1.63	2.94
Body height	140	58.50	64.00	61.49	1.65	2.68
	180	62.00	67.00	64.66	1.57	2.43
体长/cm	100	55.50	61.34	58.36	1.66	2.85
Body length	140	63.00	68.50	65.73	1.70	2.59
	180	68.00	73.50	70.69	1.61	2.28
胸围/cm	100	59.50	65.00	62.25	1.77	2.84
Chest circumference	140	68.50	74.00	71.49	1.69	2.37
	180	78.00	83.00	80.50	1.72	2.13
初生质量/kg Birth weight	1	2.60	6.80	4.05	0.76	18.80
断奶质量/kg Weaning weight	56	11.80	17.70	14.49	1.64	11.31
饲料转化率/%	100~140	4.08	9.44	5.70	0.87	15.30
Feed conversion rate	140~180	3.41	9.11	6.96	1.08	15.57
胴体质量/kg Carcass weight	180	20.80	26.70	23.63	1.73	7.34
宰前活质量/kg Live weight before slaughter	180	41.20	47.10	43.83	1.71	3.91
屠宰率/% Dressing percentage	180	0.50	0.56	0.53	0.02	3.08
眼肌面积/cm² Eye muscle area	180	8.95	14.92	11.86	1.46	12.29

位点 Loci	基因型 Genotype	个数 Number	基因型频率 Genotype frequency	等位基因 Allele	等位基因频率 Allele frequency	Ne	Ho	He	PIC	HWE-χ²
g.4039718C>T	TT	64	0.06	T	0.28
	CT	424	0.43			1.68	0.6	0.4	0.32	0.59
	CC	511	0.51	C	0.72

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湖羊CEL基因多态性及其与眼肌面积性状的关联分析

Analysis of CEL Gene Polymorphisms and Their Association with Eye Muscle Area Traits in Hu Sheep

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性状 Traits	TT(64)	CT(424)	CC(511)
眼肌面积 Eye muscle area	13.175±0.463a	12.001±0.169b	12.011±0.140b
胴体背膘厚 Carcass with thick backfat	0.941±0.102a	1.085±0.038a	1.069±0.039a
胴体质量 Carcass weight	24.848±0.679a	24.103±0.271a	23.986±0.247a
屠宰率 Slaughter rate	0.541±0.004a	0.538±0.001a	0.535±0.001a
宰前活质量 Live weight before slaughter	45.769±1.101a	44.544±0.440a	44.611±0.401a

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