陆地棉细胞色素P450基因<i>GhP450-94C1</i>黄萎病抗性功能验证

doi:10.7668/hbnxb.20192979

摘要/Abstract

摘要：

为了探究陆地棉细胞色素P450基因GhP450-94C1在棉花黄萎病反应中的生物学功能,为棉花黄萎病抗性基因挖掘及抗病育种奠定基础,通过转录组筛选,克隆获得一个细胞色素P450基因GhP450-94C1,利用生物信息学的方法分析该基因的理化性质,通过荧光定量PCR技术分析GhP450-94C1在黄萎病侵染下的表达模式,利用病毒诱导的基因沉默(VIGS)技术初步探究其在棉花抗黄萎病反应中的生物学功能。结果表明,克隆获得一个陆地棉细胞色素P450基因GhP450-94C1,其开放阅读框(ORF)为1 503 bp,编码一个含500个氨基酸的酸性、亲水、不稳定的跨膜蛋白,分子式为C₂₅₉₇H₄₀₂₅N₆₉₁O₇₂₅S₂₂,分子质量为57.23 ku,定位于内质网膜,含有一个P450结构域;有86.45%的概率存在信号肽;二级结构预测显示,该蛋白含有24个α-螺旋和8个β-折叠;该基因响应黄萎病菌侵染,抑制其表达后植株对黄萎病菌的敏感性增强。初步证明GhP450-94C1是棉花黄萎病抗性反应的一个正向调控因子。

关键词: 棉花黄萎病, GhP450-94C1, 基因克隆, 病毒诱导的基因沉默

Abstract:

To explore the biological function of cotton cytochrome P450 gene GhP450-94C1 in cotton Verticillium wilt response, and lay a foundation for cotton Verticillium wilt resistance gene mining and disease resistance breeding. A cytochrome P450 gene, GhP450-94C1, was cloned through transcriptome screening. The physicochemical properties of the gene were analyzed by bioinformatics methods. The expression pattern of GhP450-94C1 under Verticillium wilt induction was analyzed by Real-time quantitative polymerase chain reaction (qRT-PCR). Virus-induced gene silencing (VIGS) technology was used to preliminarily explore its biological function in cotton resistance to Verticillium wilt. The main results were as follows: upland cotton cytochrome P450 gene, GhP450-94C1, was obtained by cloning. The open reading frame (ORF) was 1 503 bp, encoding an acidic, hydrophilic and unstable transmembrane protein with 500 amino acids. The molecular formula was C₂₅₉₇H₄₀₂₅N₆₉₁O₇₂₅S₂₂ with a molecular weight of 57.23 ku, which was located in the endoplasmic reticulum membrane and contained a P450 domain. There was 86.45% probability of signal peptide; the secondary structure prediction showed that the protein contained 24 α-helixes and 8 β-sheets. This gene responds to Verticillium wilt infection, and after inhibiting its expression, the sensitivity of plants to Verticillium wilt is enhanced. GhP450-94C1 is a positive regulator of cotton resistance to Verticillium wilt.

Key words: Cotton Verticillium wilt, GhP450-94C1, Gene cloning, Virus-induced gene silencing

胡子曜, 李秀青, 代培红, 雷建峰, 柳建飞, 赵燚, 邓嘉辉, 刘超, 刘晓东, 李月. 陆地棉细胞色素P450基因GhP450-94C1黄萎病抗性功能验证[J]. 华北农学报, 2022, 37(6): 72-81. doi: 10.7668/hbnxb.20192979.

HU Ziyao, LI Xiuqing, DAI Peihong, LEI Jianfeng, LIU Jianfei, ZHAO Yi, DENG Jiahui, LIU Chao, LIU Xiaodong, LI Yue. Functional Verification of GhP450-94C1 that a Verticillium wilt Resistant Gene in Gossypium hirsutum L.[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(6): 72-81. doi: 10.7668/hbnxb.20192979.

http://www.hbnxb.net/CN/Y2022/V37/I6/72

图/表 10

表1 生物信息学分析工具

Tab.1 Bioinformatics analysis tools and corresponding functions

分析工具 Analysis tools	功能 Function	网址 URL
ExPASy-ProtScale	蛋白理化性质分析	http://web.expasy.org/protparam/
SignalP	信号肽预测	http://www.cbs.dtu.dk/services/SignalP
TMHMM	跨膜结构域预测	http://www.cbs.dtu.dk/services/TMHMM/
LocTree	亚细胞定位分析	https://www.rostlab.org/services/loctree2/
SMART	蛋白结构域预测	http://smart.embl-heidelberg.de/
PSIPRED	蛋白二级结构预测	http://bioinf.cs.ucl.ac.uk/psipred/
SWISS-MODEL	蛋白三级结构预测	https://swissmodel.expasy.org/

表2 引物及用途

Tab.2 Primer and application

引物名称 Primer name	正向引物(5'—3') Forward primer(5'—3')	反向引物(5'—3') Reverse primer(5'—3')	用途 Application
GhP450-94C1	ATGGATTCACAACTTTCCTTG	TTAAGCTTCCCTTTCTTGGAC	基因克隆
q-GhP450-94C1	GTCACCTACCATCCATATGC	CACCAATGCTATTTCCTTCCC	qRT-PCR
q-GhUBQ7	GAAGGCATTCCACCTGACCAAC	CTTGACCTTCTTCTTCTTGTGCTTG	qRT-PCR
q-GhCLA1	GCCCTTTGTGCATCTTC	CTCTAGGGGCATTGAAG	qRT-PCR
CM-GhP450-94C1	GAATTCGATTTCGATAATCTTTGTGATTGG	GGTACCATTCGAGGCAACCAGGGTC	载体构建

图1 GhP450-94C1基因的克隆 M.Trans 2000 PlusⅡ DNA Marker。图6同。

Fig.1 Cloning of GhP450-94C1 gene M.Trans 2000 PlusⅡ DNA Marker.The same as Fig.6.

图2 GhP450-94C1蛋白结构域预测

Fig.2 The prediction protein domain of GhP450-94C1

图3 GhP450-94C1蛋白信号肽预测

Fig.3 The prediction signal peptide of GhP450-94C1 protein

图4 GhP450-94C1蛋白的二、三级结构预测 A.GhP450-94C1蛋白的二级结构预测;B.GhP450-94C1蛋白的三级结构预测。

Fig.4 The prediction secondary and tertiary structure of GhP450-94C1 protein A.The prediction secondary structure of GhP450-94C1 protein;B.The prediction tertiary structure of GhP450-94C1 protein.

图5 黄萎病菌侵染下GhP450-94C1基因的表达模式分析相同小写字母表示差异不显著(P>0.05),不同小写字母之间表示差异显著(P<0.05)。图7—8同。

Fig.5 Analysis of the expression patterns of GhP450-94C1 under the treatment of Verticillium wilt The same lowercase letter indicates no significant difference(P>0.05),and different lowercase letters indicate significant difference(P<0.05).The same as Fig.7—8.

图6 GhP450-94C1的VIGS载体的构建 A.GhP450-94C1的VIGS载体构建示意图;B.GhP450-94C1目标片段的PCR扩增;C.TRV∶GhP450-94C1载体的酶切鉴定。

Fig.6 Construction of VIGS vector of GhP450-94C1 A.Construction of VIGS vector of GhP450-94C1;B.PCR amplification of target fragment of GhP450-94C1;C.Identification of TRV∶GhP450-94C1 vectors by restriction enzyme digestion.

图7 GhP450-94C1基因的沉默效率检测 A.沉默GhCLA1的表型;B、C.GhCLA1和GhP450-94C1的沉默效率检测。

Fig.7 Silencing efficiency of GhP450-94C1 gene A.The phenotype of silenced GhCLA1;B,C.Silencing efficiency of GhCLA1 and GhP450-94C1.

图8 GhP450-94C1基因沉默植株的抗病性鉴定 A.GhP450-94C1基因沉默后植株的表型分析;B.病情指数统计;C.剖秆鉴定;D.病原菌恢复培养。

Fig.8 Disease resistance identification of GhP450-94C1 gene silencing plants A.Phenotype analysis of plants after GhP450-94C1 gene silencing;B.Disease index statistics;C.Stem cutting phenotype;D.Restoration culture of pathogenic bacteria.

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[42]	张翼, 邵冬南, 薛飞, 李虎情, 王学峰, 李艳军, 张新宇, 刘峰, 孙杰. 陆地棉细胞色素P450超家族基因GhCYP85A2-1的克隆与功能分析[J]. 新疆农业科学, 2021, 58(2):197-205.doi:10.6048/j.issn.1001-4330.2021.02.001. doi: 10.6048/j.issn.1001-4330.2021.02.001
	Zhang Y, Shao D N, Xue F, Li H Q, Wang X F, Li Y J, Zhang X Y, Liu F, Sun J. Cloning and functional analysis of cytochrome P450 superfamily gene GhCYP85A2-1 in upland cotton[J]. Xinjiang Agricultural Sciences, 2021, 58(2):197-205.
[43]	辛珊, 李榕, 谢全亮, 寇伟, 李鸿彬. 棉纤维细胞色素单加氧酶基因GhCYP714A1促进活性氧的累积[J]. 石河子大学学报(自然科学版), 2017, 35(1):65-69.doi:10.13880/j.cnki.65-1174/n.2017.01.011. doi: 10.13880/j.cnki.65-1174/n.2017.01.011
	Xin S, Li R, Xie Q L, Kou W, Li H B. Overexpression of a GhCYP714A1 gene encoding cytochrome P450 gene promotes the H₂O₂ accumulation in transgenic tobacco plant[J]. Journal of Shihezi University (Natural Science), 2017, 35(1):65-69.
[44]	Gu L J, Dou L L, Guo Y N, Wang H T, Li L B, Wang C C, Ma L, Wei H L, Yu S X. The WRKY transcription factor GhWRKY27 coordinates the senescence regulatory pathway in upland cotton (Gossypium hirsutum L.)[J]. BMC Plant Biology, 2019, 19(1):116.doi:10.1186/s12870-019-1688-z. doi: 10.1186/s12870-019-1688-z
[45]	Sun L Q, Zhu L F, Xu L, Yuan D J, Min L, Zhang X L. Cotton cytochrome P450 CYP82D regulates systemic cell death by modulating the octadecanoid pathway[J]. Nature Communications, 2014, 5(1):5372.doi:10.1038/ncomms6372. doi: 10.1038/ncomms6372 URL

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陆地棉细胞色素P450基因GhP450-94C1黄萎病抗性功能验证

Functional Verification of GhP450-94C1 that a Verticillium wilt Resistant Gene in Gossypium hirsutum L.

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陆地棉细胞色素P450基因GhP450-94C1黄萎病抗性功能验证

Functional Verification of GhP450-94C1 that a Verticillium wilt Resistant Gene in Gossypium hirsutum L.

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