外源油菜素内酯对低温胁迫下玉米发芽及幼苗生理特性的影响

doi:10.7668/hbnxb.201751362

摘要/Abstract

摘要： 为探究外源油菜素内酯（BR）对低温胁迫下玉米发芽及幼苗生理特性的影响，以天农九号和天和1号玉米杂交种为试验材料，分别用BR浸种和喷施处理后，对2个玉米品种低温处理下的农艺性状和生理特性进行了比较研究。结果表明，外源BR处理能有效缓解低温胁迫对玉米生长的抑制程度。其中0.1 mg/L BR处理效果最好，主要表现在玉米发芽率、幼苗地上及地下部生物量和SPAD值的增加；MDA含量和相对电导率的降低；SOD、POD和CAT活性的增加；脯氨酸、可溶性糖和可溶性蛋白含量的增加；与低温对照比较，玉米杂交种天农九号和天和1号起始发芽率分别提高18.89，11.10百分点，终发芽率分别提高6.67，18.89百分点；地上部干质量增加55.92%，49.15%，地下部干质量增加38.79%，14.26%，相对电导率降低7.33%，6.80%，CAT活性增加110.58%，120.43%，脯氨酸含量增加66.59%，54.63%。说明低温胁迫下喷施适宜浓度的BR对2个玉米品种缓解抑制能力不同，表现为天农九号 > 天和1号；抗冷性强弱不同的玉米品种对不同浓度BR的适应性存在差异。

关键词: 玉米, 低温胁迫, 油菜素内酯, 发芽, 生理特性

Abstract: In order to explore the effects of brassinolide(BR) on seed germination and seedling physiological characteristics of maize under low temperature stress, the maize hybrids Tiannong 9 and Tianhe 1 were used as the materials to study the changes of agronomic traits and physiological properties after their seeds were soaked or sprayed with BR. The results showed that exogenous BR treatment could effectively alleviate the inhibition of maize growth under low temperature stress. The best treating concentration was 0.1 mg/L BR, which increased the germination rate, the biomass of overground and underground parts, the SPAD value, the activities of SOD, POD and CAT, and the contents of proline, soluble sugar and soluble protein, while decreased the MDA content and the relative conductivity in maize seedlings. Compared with the low temperature control, the maize hybrids Tiannong 9 and Tianhe 1 treated with exogenous BR of 0.1 mg/L initial germination rate increased by 18.89 and 11.10 percentage points respectively, the final germination rate increased by 6.67 and 18.89 percentage points, their aboveground dry weight increased by 55.92% and 49.15%, their underground dry weight raised by 38.79% and 14.26%, their relative electrical conductivity decreased by 7.33% and 6.80%, their CAT activity increased by 110.58% and 120.43%, and their proline content increased by 66.59% and 54.63%, respectively. It suggested that the relieving effects of 0.1 mg/L BR treatment on the two maize varieties under low temperature stress were different, Tiannong 9 being better than Tianhe 1. The maize varieties with different cold resistances had different adaptability to different BR concentrations.

Key words: Maize, Chilling resistance, Brassinolide, Germinate, Physiological property

中图分类号:

S513.01

孙玉珺, 吴玥, 马德志, 吕金莹, 贺韵涵, 宫磊, 刘哲, 高丽丹, 李娜, 闫冬, 朱晶桓, 杨德光. 外源油菜素内酯对低温胁迫下玉米发芽及幼苗生理特性的影响[J]. 华北农学报, 2019, 34(3): 119-128. doi: 10.7668/hbnxb.201751362.

SUN Yujun, WU Yue, MA Dezhi, LÜ Jinying, HE Yunhan, GONG Lei, LIU Zhe, GAO Lidan, LI Na, YAN Dong, ZHU Jinghuan, YANG Deguang. Effects of Brassinolide on Seed Germination and Seedling Physiology of Maize under Low Temperature Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 119-128. doi: 10.7668/hbnxb.201751362.

http://www.hbnxb.net/CN/Y2019/V34/I3/119

参考文献

[1] Farooqi M Q,Sa K J,Hong T K,Lee J K. Bulk segregant analysis(BSA) for improving cold stress resistance in maize using SSR markers[J]. Genet Mol Res,2016,15:1-12.doi:10.4238/gmr15049326.
[2] Li Z,Xu J G,Gao Y E,Wang C,Guo G A,Huang Y T,Hu W M,Sheteiwy M S,Hu J. The synergistic priming effect of exogenous salicylic acid and H₂O₂on chilling tolerance enhancement during maize(Zea mays L.) seed germination[J].Frontiers in Plant Science,2017,8:1153.doi:10.3389/fpls.2017.01153.
[3] Krishna P,Prasad B D,Rahman T. Brassinosteroid action in plant abiotic stress tolerance[J]. Methods in Molecular Biology,2017,1564:193.doi:10.1007/978-1-4939-6813-8_16.
[4] 温万里,郑颖坤,艾莉,刘洪亮,孔伟庆,艾佳,杨德光,张志武. 玉米抗冷性研究进展[J]. 作物杂志,2014(4):16-21.doi:10.3969/j.issn.1005-0906.2013.04.
Wen W L,Zheng Y K,Ai L,Liu H L,Kong W Q,Ai J,Yang D G,Zhang Z W. Research progress on cold resistance of maize[J]. Crops,2014(4):16-21.
[5] Seydpour F,Sayyari M. Chilling injury in cucumber seedlings amelioration by methyl salicylate[J]. 2016,22(5):432-441.doi:10.1080/19315260.2015.1067938.
[6] Sasse J M. Physiological actions of brassinosteroids:An update[J].Journal of Plant Growth Regulation,2003,22(4):276-288.doi:10.1007/s00344-003-0062-3.
[7] Clouse S D,Sasse J M. Brassinosteroids:essential regulators of plant growth and development[J].Annual Review of Plant Physiology and Plant Molecular Biology,1998,49(49):427-451.doi:10.1146/annurev.arplant.49.1.427.
[8] You J,Chan Z L.ROS regulation during abiotic stress responses in crop plants[J].Frontiers in Plant Science,2015,6:1092.doi:10.3389/fpls.2015.01092.
[9] Burbulis N,Jonytien e·V,Kuprien e·R,Blinstrubien e·A. Changes in proline and soluble sugars content during cold acclimation of winter rapeseed shoots in vitro[J].Journal of Food Agriculture & Environment,2011,99(22):371-374.
[10] Ashraf M,Foolad M R.Roles of glycine betaine and proline in improving plant abiotic stress resistance[J].Environmental and Experimental Botany,2007,59(2):206-216.doi:10.1016/j.envexpbot.2005.12.006.
[11] Hamada K. Brassinolide:some effects for crop cultivations[C]//Conf Proc Int Seminar Plant Growth Regul.Tokyo,1986:188-196.
[12] Singh I S,Shono M. Physiological and molecular effects of 24-epibrassinolide,a brassinosteroid on thermotolerance of tomato[J].Plant Growth Regulation,2005,47(2/3):111.doi:10.1007/s10725-005-3252-0.
[13] Thussagunpanit J,Jutamanee K,Kaveeta L A,Pankean P,Homvisasevongsa S A. Comparative effects of brassinosteroid and brassinosteroid mimic on improving photosynthesis,lipid peroxidation,and rice seed set under heat stress[J].Journal of Plant Growth Regulation,2015,34(2):320-331.doi:10.1007/s00344-014-9467-4.
[14] Li J,Yang P,Gan Y T,Yu J H,Xie J M. Brassinosteroid alleviates chilling-induced oxidative stress in pepper by enhancing antioxidation systems and maintenance of photosystem Ⅱ[J].Acta Physiologiae Plantarum,2015,37(11):1-11.doi:10.1007/s11738-015-1966-9.
[15] Wu X X,Ding H D,Chen J L,Zhu Z W,Zha D S. Amelioration of oxidative damage in Solanum melongena seedlings by 24-epibrassinolide during chilling stress and recovery[J].Biologia Plantarum,2015,59(2):350-356.doi:10.1007/s10535-015-0495-0.
[16] Hirai K,Fujii S,Honjo K.Plant-growth regulating action of brassinolide.1.
the effect of brassinolide on the ripening of rice plants under the low-temperature condition[J].Japanese Journal of Crop Science,1991,60(1):29-35.doi:10.1626/jcs.60.29.
[17] 李淑叶,马慧娟,张思平,刘绍东,沈倩,陈静,葛常伟,庞朝友,赵新华. 外源2,4表油菜素内酯对低温胁迫下棉花幼苗光合生理的影响[J]. 棉花学报,2018,30(3):252-260.
Li S S,Ma H J,Zhang S P,Liu S D,Shen Q,Chen J,Ge C W,Pang C Y,Zhao X H. Effects of exogenous 2,4-epibrassinolide on photosynthetic physiology of cotton seedlings under low temperature[J]. Cotton Science,2018,30(3):252-260.
[18] 王学奎,黄见良. 植物生理生化实验原理与技术[M]. 北京:高等教育出版社,2015.
Wang X K,Huang J L. Principles and techniques of plant physiological biochemical experiment[M]. Beijing:Higher Education Press,2015
[19] Giannopolitis C N,Ries S K.Superoxide dismutases:I. Occurrence in higher plants[J].Plant Physiology,1977,59(2):309-314.doi:10.2307/4264724.
[20] Xu T. Bioaccumulation,subcellular,and molecular localization and damage to physiology and ultrastructure in Nymphoides peltata (Gmel.) O. Kuntze exposed to yttrium[J]. Environmental Science & Pollution Research,2014,21(4):2935-2942.doi:10.1007/s11356-013-2246-0.
[21] Alejandro Perdomo J,Conesa M A,Medrano H A,Galmes J. Effects of long-term individual and combined water and temperature stress on the growth of rice,wheat and maize:relationship with morphological and physiological acclimation[J].Physiologia Plantarum,2015,155(2):149-165.doi:10.1111/ppl.12303.
[22] Hatfield J L,Prueger J H. Temperature extremes:Effect on plant growth and development[J].Weather and Climate Extremes,2015,10(A,SI):4-10.doi:10.1016/j.wace.2015.08.001.
[23] Duncan W G,Hesketh J D.Net photosynthetic rates,relative leaf growth rates,and leaf numbers of 22 races of maize grown at eight temperatures 1[J].Crop Science,1968,8(6):670-674.doi:10.2135/cropsci1968.0011183X000800060009x.
[24] Muldoon D K,Wheeler J L,Pearson C J.Growth,mineral-composition and digestibility of maize,sorghum and barnyard millets at different temperatures[J].Australian Journal of Agricultural Research,1984,35(3):367-378.doi:10.1071/AR9840367.
[25] Yildirim E,Karlidag H,Dursun A.Salt tolerance of physalis during germination and seedling growth[J].Pakistan Journal of Botany,2011,43(6):2673-2676.doi:10.1.1.546.8972.
[26] 刘禹辰,杨德光,李梁,柴孟竹,鞠然,白冰,刘宇龙. 低温胁迫对玉米种子萌发及淀粉分解酶类活性的影响1[J]. 玉米科学,2017,26(1):64-68.doi:10.13597/j.cnki.maize.science.20180110.
Liu Y C,Yang D G,Li L,Chai M Z,Ju R,Bai B,Liu Y L. Effect of low temperature stress on maize seed germination and starch decomposition enzyme activity[J]. Journal of Maize Science,2017,26(1):64-68.
[27] 杨德光,马月,刘永玺,杨慧,周婷婷,边鸣镝. 低温胁迫下外源CaCl₂对玉米种子萌发及幼苗生长的影响[J]. 玉米科学,2018,26(3):83-88.doi:10.13597/j.cnki.maize.science.20180314.
Yang D G,Ma Y,Liu Y X,Yang H,Zhou T T,Bian M D. Effects of exogenous CaCl₂ on maize seed germination and seeding growth under low temperature stress[J]. Journal of Maize Sciences,2018,26(3):83-88.
[28] 杨德光,孙玉珺,Irfan A R,刘昕萌,吕金莹,尉菊萍,宫磊,刘哲,白冰. 低温胁迫对玉米发芽及幼苗生理特性的影响[J]. 东北农业大学学报,2018,49(5):1-8,44.doi:10.19720/j.cnki.issn.1005-9369.2018.05.001.
Yang D G,Sun Y J,Irfan A R,Liu X M,Lü J Y,Wei J P,Gong L,Liu Z,Bai B. Effect of low temperature stress on germination and physiological of maize seedling[J]. Journal of Northeast Agricultural University,2018,49(5):1-8,44.
[29] 马金虎. 油菜素内酯调控低温胁迫下玉米种子萌发的生理机制[D]. 晋中:山西农业大学,2015.
Ma J H. Physiolgical mechanism of EBR promotr low temperature stress in maize seed germination[D].Jinzhong:Shanxi Agricultural University,2015.
[30] Engels C. Effect of root and shoot meristem temperature on shoot to root dry matter partitioning and the internal concentrations of nitrogen and carbohydrates in maize and wheat[J].Annals of Botany,1994,73(2):211-219.doi:10.1006/anbo.1994.1025.
[31] Stone P J,Sorensen I B,Jamieson P D. Effect of soil temperature on phenology,canopy development,biomass and yield of maize in a cool-temperate climate[J].Field Crops Research,1999,63(2):169-178.doi:10.1016/S0378-4290(99) 00033-7.
[32] Sowiński P,Rudzińskalangwald A,Adamczyk J,Kubica I,Fronk J.Recovery of maize seedling growth,development and photosynthetic efficiency after initial growth at low temperature[J].Journal of Plant Physiology,2004,162(1):67-80.doi:10.1016/j.jplph.2004.03.006.
[33] Rymen B,Fiorani F,Kartal F,Vandepoele K,Inze D,Beemster G T. Cold nights impair leaf growth and cell cycle progression in maize through transcriptional changes of cell cycle genes[J].Plant Physiology,2007,143(3):1429-1438.doi:10.1104/pp.106.093948.
[34] 徐晓昀,郁继华,颉建明,胡琳莉,李杰. 水杨酸和油菜素内酯对低温胁迫下黄瓜幼苗光合作用的影响[J]. 应用生态学报,2016,27(9):3009-3015.doi:10.13287/j.1001-9332.201609.020.
Xu X Y,Yu J H,Jie J M,Hu L L,Li J. Effects of exogenous salicylic acid and brassinolide on photosynthesis of cucumber seedlings under low temperature stress[J]. Chinese Journal of Applied Ecology,2016,27(9):3009-3015.
[35] 刘巾瑞. 表油菜素内酯对高粱低温胁迫的缓解作用研究[D]. 大庆:黑龙江八一农垦大学,2018.
Liu J R. Study on the alleviating effect of epibrassinolide on low temperature stress of sorghum[D].Daqing:Heilongjiang Bayi Agricultural University,2018.
[36] 张小贝,祝志欣,南文卓,孙言博,朱国鹏. 2,4-表油菜素内酯(EBR) 对菜用甘薯抗寒生理生化的影响[J]. 安徽农业大学学报,2017,44(3):525-529.doi:10.13610/j.cnki.1672-352x.20170524.027.
Zhang X B,Zhu Z X,Nan W Z,Sun Y B,Zhu G P. Effects of 2,4-epibrassinolide(EBR) on cold resistance-related physiological-biochemical indexes of vegetable sweet potato[J]. Journal of Anhui Agricultural University,2017,44(3):525-529.
[37] 周爱清,骆炳山,任筱波. 油菜素内酯对水稻幼苗生长及耐冷性的影响[J]. 华中农业大学学报,1987(1):8-13.doi:10.13300/j.cnki.hnlkxb.1987.01.002.
Zhou A Q,Luo B S,Ren X B. Effcet of brassinolide on rice seedling growth and cold resistance[J]. Journal of Huazhong Agricultural,1987(1):8-13.
[38] Mauch-Mani B,Mauch F.The role of abscisic acid in plant pathogen interactions[J].Current Opinion in Plant Biology,2005,8(4):409-414.doi:10.1016/j.pbi.2005.05.015.
[39] Wise R R,Naylor A W. Chilling-Enhanced photooxidation:the peroxidative destruction of lipids during chilling injury to photosynthesis and ultrastructure[J].Plant Physiology,1987,83(2):272-277.doi:10.1104/pp.83.2.272.
[40] Wang Y,Zhou M,Gong X L,Liu C,Hong M M,Wang L,Hong F S. Influence of lanthanides on the antioxidative defense system in maize seedlings under cold stress[J].Biological Trace Element Research,2011,142(3):819-830.doi:10.1007/s12011-010-8814-y.
[41] Allen D J,Ort D R.Impacts of chilling temperatures on photosynthesis in warm-climate plants[J].Trends in Plant Science,2001,6(1):36-42.doi:10.1016/s1360-1385(00) 01808-2.
[42] Wu X X,Ding H D,Chen J L,Zhu Z W,Zha D S. Exogenous spray application of 2,4-epibrassinolide induced changes in photosynthesis and anti-oxidant defences against chilling stress in eggplant(Solanum melongena L.) seedlings[J].Journal of Horticultural Science & Biotechnology,2015,90(2):217-224.doi:10.1080/14620316.2015.11513175.
[43] Machado D P,Ribeiro R V,Silveira J G,Filho J M,Machado E C. Rootstocks induce contrasting photosynthetic responses of orange plants to low night temperature without affecting the antioxidant metabolism[J].Theoretical and Experimental Plant Physiology,2013,25(1):26-35.
[44] Gapper C,Dolan L. Control of plant development by reactive oxygen species[J].Plant Physiology,2006,141(2):341-345.doi:10.1104/pp.106.079079.
[45] Zhou B Y,Guo Z F,Xing J P,Huang B R.Nitric oxide is involved in abscisic acid-induced antioxidant activities in Stylosanthes guianensis[J].Journal of Experimental Botany,2005,56(422):3223-3228.doi:10.1093/jxb/eri319.
[46] Ahammed G J,Yuan H L,Ogweno J O,Zhou Y H,Xia X J,Mao W H,Shi K,Yu J Q. Brassinosteroid alleviates phenanthrene and pyrene phytotoxicity by increasing detoxification activity and photosynthesis in tomato[J].Chemosphere,2012,86(5):546-555.doi:10.1016/j.chemosphere.2011.10.038.
[47] Liu Y J,Zhao Z G,Si J,Di C X,Han J,An L Z. Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana[J].Plant Growth Regulation,2009,59(3):207-214.doi:10.1007/s10725-009-9405-9.
[48] Fariduddin Q,Yusuf M,Chalkoo S,Hayat S,Ahmad A.28-homobrassinolide improves growth and photosynthesis in Cucumis sativus L. through an enhanced antioxidant system in the presence of chilling stress[J].Photosynthetica,2011,49(1):55-64.doi:10.1007/s11099-011-0022-2.

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