小麦<em>TaWRKY71a</em>基因克隆、生物信息学及表达分析

doi:10.7668/hbnxb.2018.03.002

华北农学报 ›› 2018, Vol. 33 ›› Issue (3): 7-13. doi: 10.7668/hbnxb.2018.03.002

所属专题： 小麦；生物技术；

小麦TaWRKY71a基因克隆、生物信息学及表达分析

王俊斌^1,2, 杨文丽¹, 丁博¹, 吴天文¹, 王海凤³, 谢晓东¹

1. 天津农学院天津-布里斯托环境变化对农作物影响研究中心, 天津 300384;
2. 天津农学院基础科学学院, 天津 300384;
3. 天津农学院农业分析测试中心, 天津 300384

收稿日期:2017-12-28 出版日期:2018-06-28
通讯作者: 谢晓东(1975-),男,内蒙古兴安盟人,研究员,博士,主要从事作物遗传育种研究。
作者简介:王俊斌(1979-),男,内蒙古呼和浩特人,副研究员,博士,主要从事植物抗逆机制研究。
基金资助:
天津市应用基础与前沿技术研究计划项目（14JCYBJC30600）；国家自然科学基金项目（31671611）

Cloning,Bioinformatics and Expression Analysis of TaWRKY71a Gene in Wheat

WANG Junbin^1,2, YANG Wenli¹, DING Bo¹, WU Tianwen¹, WANG Haifeng³, XIE Xiaodong¹

1. Tianjin-Bristol Research Center for the Effects of the Environment Change on Crops, Tianjin Agricultural University, Tianjin 300384, China;
2. College of Basic Sciences, Tianjin Agricultural University, Tianjin 300384, China;
3. Center for Agricultural Analysis and Measurement, Tianjin Agricultural University, Tianjin 300384, China

Received:2017-12-28 Published:2018-06-28

摘要/Abstract

摘要： WRKY蛋白是主要存在于植物体内一类转录因子家族，在植物的生长发育和逆境应答中发挥重要作用。为了揭示小麦WRKY基因功能，从普通小麦中克隆出TaWRKY71a基因后进行氨基酸序列分析，并对TaWRKY71a基因在不同组织及不同胁迫条件下的表达模式进行分析。结果表明，TaWRKY71a基因编码一个含有355个氨基酸的蛋白质。氨基酸序列分析显示，TaWRKY71a蛋白含有1个WRKY结构域及1个C₂H₂锌指结构。预测分析表明，TaWRKY71a蛋白定位在细胞核；其二级结构包含28.17%的α-螺旋、16.34%的延伸链、4.79%的β-转角和50.70%的无规则卷曲。不同物种间WRKY蛋白的同源性分析比较表明，TaWRKY71a与粗山羊草WRKY71、水稻WRKY71、高粱WRKY71和拟南芥WRKY40的氨基酸序列之间具有高度的保守性。qRT-PCR分析表明，TaWRKY71a在叶、根、茎、花和种子中均有不同程度表达，并受ABA、NaCl和PEG诱导表达，推测该基因可能参与小麦逆境胁迫应答。研究结果为进一步研究TaWRKY71a的生物学功能奠定了基础。

关键词: 小麦, TaWRKY71a基因, 克隆, 基因表达, 生物信息学

Abstract: WRKY proteins are a large super family of transcription factors(TFs)and exist mainly in plants. It has been well documented that WRKY TFs play important roles in plant growth and development,and responses to stresses. In order to deeply understand the gene function of WRKY,a novel WRKY gene,named TaWRKY71a, was isolated from common wheat. The coding sequence of this gene was predicted with the bioinformatics analysis. Furthermore,qRT-PCR assay was performed to investigate the tissue-specific expression of TaWRKY71a,and its expressed patterns under various abiotic stresses and signaling molecules treatments. The results showed that TaWRKY71a gene encoded a protein with 355 amino acid residues. Sequence analysis showed that TaWRKY71a contained one WRKY domain and C₂H₂ zinc-finger structure. Based on bioinformatics analysis,TaWRKY71a was located in the nucleus. The potential secondary structure for the protein included about 28.17% alpha helixes,16.34% extended strand,4.79% beta turn and 50.70% random coil as predicted with softwares. The phylogenic tree indicated that TaWRKY71a shared high similarity to WRKY71 from Aegilops tauschii, Oryza sativa, Sorghum bicolor and WRKY40 from Arabidopsis. TaWRKY71a expressed differentially in various organs such as leaves,roots,stems,flowers and seeds of wheat. After treatment with ABA,NaCl and PEG, TaWRKY71a showed higher expression levels than control. The fact implied that it might be involved in stress signaling pathways. The results will provide a basis for further functional studies of TaWRKY71a.

Key words: Wheat, TaWRKY71a gene, Cloning, Gene expression, Bioinformatics

中图分类号:

Q785
S512.03

王俊斌, 杨文丽, 丁博, 吴天文, 王海凤, 谢晓东. 小麦TaWRKY71a基因克隆、生物信息学及表达分析[J]. 华北农学报, 2018, 33(3): 7-13. doi: 10.7668/hbnxb.2018.03.002.

WANG Junbin, YANG Wenli, DING Bo, WU Tianwen, WANG Haifeng, XIE Xiaodong. Cloning,Bioinformatics and Expression Analysis of TaWRKY71a Gene in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 7-13. doi: 10.7668/hbnxb.2018.03.002.

http://www.hbnxb.net/CN/Y2018/V33/I3/7

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