荣昌猪骨形态发生蛋白15基因克隆与表达谱分析

doi:10.7668/hbnxb.2016.S1.078

摘要/Abstract

摘要： 骨形态发生蛋白15（Bone morphogenetic protein 15，BMP15）由卵母细胞特异高表达，在卵母细胞发育成熟及动物繁殖性能具有重要的调控作用。通过采集荣昌猪卵泡组织，克隆BMP15基因并对其mRNA组织表达谱进行分析。研究显示：荣昌猪BMP15基因片段含有完整开放阅读框，与野猪、长白猪及梅山猪BMP15基因进化关系最为密切，具有转化生长因子β（Transforming growth factor-beta，TGF-β）超家族成员结构特征，具有卵巢组织特异性高表达特征。这些研究结果为进一步探究BMP15基因的表达调控机制及其在猪卵泡发育中的作用奠定基础。

关键词: 荣昌猪, 骨形态发生蛋白, 克隆, 转录谱

Abstract: Bone morphogenetic protein-15(BMP15) was exclusively expressed in oocyte and plays important role in oocyte development and maturation in vertebrate. In this study,using rongchang pig follicle tissue,the BMP15 gene was cloned and its mRNA transcripts expression was analyzed. The studies showed that Rongchang pig BMP15 (rcBMP15) gene contained a 1 298 bp in length and containing complete open reading frame and was most close to wild boar,landrace and meishan pigs BMP15 gene in evolutionary relationships. rcBMP15 gene had typical structure characteristics of the transforming growth factor beta family. Using RT-PCR,we also found that it is highly expressed exclusively in pig ovary. These results laid the foundation to elucidate the roles in regulation mechanism and follicular development of the BMP15 gene.

Key words: Rongchang porcine, BMP15, Cloning, Transcription profile

中图分类号:

马鲜平, 朱涛, 龙安格, 毕俊萱, 何娇, 王瑶, 易华山. 荣昌猪骨形态发生蛋白15基因克隆与表达谱分析[J]. 华北农学报, 2016, 31(S1): 458-462. doi: 10.7668/hbnxb.2016.S1.078.

MA Xianping, ZHU Tao, LONG Ange, BI Junxuan, HE Jiao, WANG Yao, YI Huashan. Sequence and Transcripts Analysis of the Bone Morphogenetic Protein 15 in Porcine of Rongchang[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 458-462. doi: 10.7668/hbnxb.2016.S1.078.

http://www.hbnxb.net/CN/Y2016/V31/IS1/458

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