摘要： 黑胫病是烟草大田生产的主要病害之一。烟草种质资源抗病性的筛选、鉴定和亲缘关系分析是抗病育种的基础。利用黑胫病1号生理小种对49份种质资源进行了抗病性鉴定，并利用SSR分子标记进行了遗传多样性和亲缘关系分析。结果表明：24份材料表现为抗黑胫病，其中大叶永烟、G80、E9、MS212-8的抗性比较好；7份材料表现为中抗黑胫病；中感黑胫病和高感黑胫病材料共6份；12份材料表现为感黑胫病。49份烟草种质资源的平均有效等位基因数为3.06（1.31），平均Shannon's信息指数为1.18（0.36）。23对SSR引物在遗传相似系数为0.66时将49份材料分为4个类群，第Ⅰ类群为抗病材料K326；第Ⅱ类群包括6份材料；第Ⅲ类群为4份感病材料；第Ⅳ类群包括38份材料。抗病材料主要集中在第Ⅳ类群，其遗传背景狭窄。

关键词: 烟草, 黑胫病, SSR, 亲缘关系

Abstract: Black shank disease is one of the main diseases of tobacco production.The screening,identification of the disease resistance and genetic analysis of tobacco germplasm resources are the base of breeding for disease resistance. In this study,we used Physiological race 1 black shank to identify the resistance to black shank of 49 tobacco germplasm resources and used SSR markers to analysis genetic relationship. The results showed:Among them 24 were resistant,including dayeyongyan,G80,E9,MS212-8;7 were moderate resistant;6 were moderate susceptible;12 were susceptible;6 were high susceptible.The average effective number of alleles of 49 tobacco germplasm resources was 3.06(1.31),and the average Shannon's information index was 1.18(0.36);The 49 germplasm materials were classed into 4 groups at the position of coefficient of 0.66 by the 23 SSR markers. The first group was resistance variety K326;the second group included 6 germplasm resources;the third group included 4 susceptible germplasm resources;the fourth group included 38 germplasm resources. The most of resistance to black shank germplasm was clustered in the fourth group,suggesting the genetic basis of the resistance to black shank germplasm resources was narrow.

Key words: Tobacco, Black shank, SSR, Genetic relationship

中图分类号: 

• S572.03