[1] Bu Y,Wang H,Li J,et al.Long distance multiple-site directed plasmid mutagenesis by one-step PCR using non-overlapped primers[J].Molecular Biotechnology,2013,55(1):49-53.
[2] 张浩,毛秉智.定点突变技术的研究进展[J].免疫学杂志,2000,16(S1):108-110.
[3] Kunkel T A.Rapid and efficient site-specific mutagenesis without phenotypic selection[J].Proceedings of the National Academy of Sciences of the United States of America,1985,82(2):488-492.
[4] Wei D,Li M,Zhang X,et al.An improvement of the site-directed mutagenesis method by combination of megaprimer,one-side PCR and DpnI treatment[J].Analytical Biochemistry,2004,331(2):401-403.
[5] Nagy Z B,Felf ldi F,Tamás L,et al.A one-tube,two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product[J].Analytical Biochemistry,2004,324(2):301-303.
[6] Chapnik N,Sherman H,Froy O,et al.A one-tube site-directed mutagenesis method using PCR and primer extension[J].Analytical Biochemistry,2007,372(2):255-257.
[7] Angelaccio S,Bonaccorsi Di Patti M C.Site-directed mutagenesis by the megaprimer PCR method:variations on a theme for simultaneous introduction of multiple mutations[J].Analytical Biochemistry,2002,306(2):346-349.
[8] Tseng W C,Lin J W,Wei T Y,et al.A novel megaprimed and ligase-free,PCR-based,site-directed mutagenesis method[J].Analytical Biochemistry,2008,375(2):376-378.
[9] 张碧乾,邓会群,宫彩霞,等.大引物PCR定点突变方法的改进[J].湖北大学学报:自然科学版,2009,31(1):79-81.
[10] Kumar R,Rajagopal K.Single-step overlap-primer-walk polymerase chain reaction for multiple mutagenesis without overlap extension[J].Analytical Biochemistry,2008,377(1):105-107.
[11] 郭子平,翟清华,曾令锋,等.小麦TaGAPC定点突变体基因载体构建及其原核表达[J].华北农学报,2016,31(1):46-50.
[12] Shan Q,Wang Y,Li J,et al.Genome editing in rice and wheat using the CRISPR/Cas system[J].Nature Protocols,2014,9(10):2395-2410.
[13] Wang H Y,Yang H,Shivalila C S,et al.One-Step Generation of mice carrying mutations in multiple genes by CRISPR/Cas-Mediated genome engineering[J].Cell,2013,153(4):910-918.
[14] Feng Z,Zhang B,Ding W,et al.Efficient genome editing in plants using a CRISPR/Cas system[J].Cell Research,2013,23(10):1229-1232.
[15] Jiang W,Zhou H,Bi H,et al.Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis,tobacco,sorghum and rice[J].Nucleic Acids Research,2013,41(20):e188.
[16] Gao J,Wang G,Ma S,et al.CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum[J].Plant Molecular Biology,2015,87(1/2):99-110.
[17] Carey M F,Peterson C L,Smale S T.PCR-mediated site-directed mutagenesis[J].Cold Spring Harbor Protocols,2013(8):738-742.
[18] Weiner M P,Costa G L.Rapid PCR site-directed mutagenesis[J].PCR Methods and Applications,1994,4(3):131-136.
[19] Rabhi I,Guedel N,Chouk I,et al.A novel simple and rapid PCR-based site-directed mutagenesis method[J].Molecular Biotechnology,2004,26(1):27-34.
[20] Li J F,Zhang D,Sheen J.Cas9-based genome editing in Arabidopsis and tobacco[J].Methods in Enzymology,2014,546:459-472. |