摘要： 选取抗TuMV的8407、河304和感TuMV的冠291和春月黄为试验材料,于苗期接种TuMV-C₄,接种后测定24 d内叶片中超氧化物岐化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)这3种过氧化氢代谢相关酶的活性以及过氧化氢H₂O₂的含量。结果表明:接种TuMV后,POD、CAT的活性及H₂O₂含量的变化在不同材料之间存在明显差异。抗病材料在接种后,POD、CAT的活性及H₂O₂含量虽有变化,但均能逐渐恢复正常;感病材料在接种后,POD、CAT的活性及H₂O₂含量均有较大变化,且始终无法恢复正常。总体而言,叶片中的和CAT与大白菜的TuMV抗性关系较为紧密,其次是POD,而SOD与TuMV抗性的关系不大。

关键词: 大白菜, 芜菁花叶病毒, 酶活性, H₂O₂

Abstract: The 4 materials 8407(R),He304(R),Guan291(S) and Chunyuehuang(S) which have different re-sistance to TuMV,were chosen to be the material plants. They were inoculated with TuMV-C₄ at seedling stage. Activi-ties of SOD,POD,CAT and H₂O₂ content from 4 materials were detected within 24 days after being inoculated with TuMV.The results showed that significant differences in the changes of enzymes activities of POD,CAT and H₂O₂ content existed in different materials. The enzymes activities of POD,CAT and H₂O₂ content in resistant materials had some changes with TuMV infection,but could gradually return to normal. The enzymes activities of POD,CAT and H₂O₂ content in susceptible materials all had significant changes,and could not return to normal after being in-oculate with TuMV. Overall TuMV resistance of Chinese cabbage had the most closely relationship with H₂O₂ and CAT,and had less relationship with POD,and had no relationship with SOD.

Key words: Chinese cabbage, TuMV, Enzyme activity, H₂O₂

中图分类号: 

• S634.1