摘要： 为揭示牛MHC-DRB3上游调控区多态对其表达调控作用,利用PCR-SSCP和DNA序列测定技术分析并建立BoLA-DRB3*exon2和BoLA-DRB3 URR之间的连锁关系。结合不同近端调控区与转录因子结合模式预测结果,选择3种不同上游调控序列的连锁组合,应用实时定量RT-PCR技术对牛MHC-DRB3基因mRNA相对表达量进行了验证分析。结果表明,上游调控区基因型为H17H17的BoLA-DRB3基因mRNA相对表达量显著高于(P<0.01)基因型H1H1及H5H6。初步证明上游调控区中的多态会引起结构基因表达量的变化。

关键词: 牛, MHC-DRB3基因, 上游调控区多态性, 实时定量RT-PCR

Abstract: To study the regulation of upstream regulatory region polymorphisms on BoLA- DRB3 gene expres- sion，PCR- SSCP and DNA sequencing were used to identify the linkage relationship between BoLA- DRB3* exon2 and BoLA- DRB3 URR．Combined with the predicted results of binding mode between different URR and transcrip- tion factor，three linkage types were selected to detect the BoLA-DRB3 mRNA levels by relative Real- time PCR ex- periments． The results showed that the expression abundances of BoLA-DRB3 varied in different linkage types.The expression level of mRNA of BoLA- DRB3 gene linked with H17H17 was significant higher than H1H1 and H5H6 (P＜0.01)．This preliminary proved that the polymorphism of upstream regulatory region can cause the expression change of BoLA- DRB3 structural gene．

Key words: Cow, BoLA- DRB3 gene, Polymorphism of URR, Real- time PCR

中图分类号: 

• Q78