摘要： 该研究以内蒙古阿尔巴斯型白绒山羊为研究对象,选择绒山羊催乳素(PRL)及其受体(PRLR)基因部分序列作为候选基因,采用PCR-SSCP分子标记技术和DNA测序技术研究其多态性,然后运用最小二乘方差分析方法,对各基因型间生产性状指标进行差异显著性检验,分析绒山羊PRL、PRLR基因的SNP与绒山羊产绒性状的关系,寻找可用于辅助选择的分子标记。结果表明:PRL基因P1和PRLR基因P3引物位点存在SSCP多态,在PRL基因Exon 5区域存在X76049:g.576 bp C-A突变,该突变导致第176个密码子CCC变成ACC,从而使脯氨酸变成苏氨酸(Pro-Thr)。方差分析表明,该位点多态与绒厚度存在极显著相关(P<0.01),CA基因型羊绒厚度比CC基因型长0.674cm;在PRLR基因Exon 10区域存在HQ266606:g.1076 bp T-C突变,该突变导致第359个密码子CTT变成CCT,从而使亮氨酸变成脯氨酸(Lue-Pro),该位点多态对绒山羊羊绒细度有显著影响(P<0.05),AA基因型羊绒细度比BB基因型细0.38μm。因此,在绒山羊羊绒厚度性状上,P1-CA基因型可以作为标记基因型;在羊绒细度性状上,P3-AA基因型可以作为标记基因型,作为绒山羊产绒性状的辅助选择分子标记位点。
关键词:
绒山羊,
催乳素(受体)基因,
单核苷酸多态性,
产绒性状
Abstract: Ardrs white cashmere goats were used as experimental sample，and partial sequence of PRL and?PRLR genes were used as candidate gene to research SNP by PCR-SSCP and DNA sequencing technologies. Results?were analyzed by least square mean (LSM) between production traits of different genotype. Relationship of SNP of?PRL and PRLR genes and production cashmere traits were studied to find molecular marker of MAS. There were SSCP polymorphism at P1 of PRL and P3 of PRLR，and existed in C-A mutation at X76049:g. 576 bp of Exon 5 of?PRL gene，which resulted in CCC-ACC of the 176thcodon from Pro into Thr. The results showed that there was the?most significant correlation with cashmere thickness at polymorphism site(P ＜0. 01)，and cashmere thickness of CA?genotype increased by 0. 674 cm compared with that of CC;there was T-C mutation at HQ266606:g Exon 10 of?PRLR gene，which lead to CTT-CCT of the 359thcodon from Lue into Pro，of which polymorphism was significant?difference (P ＜0. 05) to cashmere fineness，and cashmere fineness of AA genotype decrease by 0. 38 μm compared?with that of BB. P1-CA was the favorable marker genotype for cashmere thickness trait，while P3-AA was the marker?genotype of cashmere fineness trait，which acted as molecular marker of MAS for production cashmere traits.?
Key words:
Cashmere goat,
PRL/PRLR gene,
SNP,
Production cashmere traits
中图分类号:
刘斌, 李玉荣, 马跃军, 赵存发, 尉光荣, 李金泉. 内蒙古白绒山羊催乳素及其受体基因多态性与产绒性状的关联性分析[J]. 华北农学报, 2012, 27(2): 61-66. doi: 10.3969/j.issn.1000-7091.2012.02.012.
LIU Bin, LI Yu-rong, MA Yue-jun, ZHAO Cun-fa, WEI Guang-rong, LI Jin-quan. Correlations Between SNP of PRL/PRLR Genes and Production Cashmere Traits on Inner Mongolion White Cashmere Goat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 61-66. doi: 10.3969/j.issn.1000-7091.2012.02.012.