养分胁迫对威优916生育后期叶鞘衰老影响的蛋白质组分析

doi:10.7668/hbnxb.2011.03.022

摘要/Abstract

摘要： 从蛋白质组学角度,对养分胁迫下杂交水稻威优916早衰过程中叶鞘的蛋白质差异表达进行分析,以揭示叶鞘衰老的分子机理.水稻生育后期设清水及常规营养液培养两种处理,分4个时期提取叶鞘蛋白质,经双向电泳分离得到电泳图谱8张,应用Imagemaster 2D Elite 5.0软件对其进行蛋白质差异表达分析,共得到2个发生差异表达蛋白质点,其中19个经质谱分析功能得到鉴定.综合分析相关蛋白质的功能及其表达量变化,结果显示,养分胁迫诱导叶鞘中大量的抗性及胁迫应答蛋白质发生差异表达,叶鞘的光合性能降低,呼吸消耗增强,干物质积累减少,导致叶鞘的早衰发生.

关键词: 水稻, 养分胁迫, 叶鞘, 衰老, 蛋白质组学

Abstract: In order to reveal the molecule mechanism of rice sheath premature senescence,differential expression proteins of hybrid Weiyou916 sheath were analyzed by suing approach of plant proteomics.Rice were cultured with pure water and normal nutrient solution at late development stage.The sheath proteins were extracted from four different times respectively and investigated by 2-DE.There 8 maps were obtained and analyzed by Imagemaster 2D Elite 5.0.Software quantification showed that 27 proteins′expression abundance changed in response to nutrient stress,among which 19 proteins were identified by mass spectrometry.Functions and expression abundances of these 19 proteins were analyzed and the conclusion can be drawn from the following result: the major differential expression proteins were associated with resistance and stress response,nutrient stress led to low photosynthesis and high respiration in rice sheath,which reduced dry matter accumulation and carried sheath forward premature senescence.

Key words: Rice, Nutrient stress, Sheath, Senescence, Proteomics

中图分类号:

S312
S511.01

谢金水, 邵彩虹, 唐秀英, 刘光, 石庆华. 养分胁迫对威优916生育后期叶鞘衰老影响的蛋白质组分析[J]. 华北农学报, 2011, 26(3): 106-112. doi: 10.7668/hbnxb.2011.03.022.

XIE Jin-shui, SHAO Cai-hong, TANG Xiu-ying, LIU Guang-rong, SHI Qing-hua. Proteomics Analysis of Rice Sheath Senescence under Nutrient Stress Condition during Late Development Stage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 106-112. doi: 10.7668/hbnxb.2011.03.022.

http://www.hbnxb.net/CN/Y2011/V26/I3/106

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