奶牛乳腺上皮细胞的体外培养方法及其应用概述

doi:10.7668/hbnxb.2010.S2.008

摘要/Abstract

摘要： 细胞的分离培养是细胞生物学的基础技术,它被广泛应用于细胞生物学、分子生物学和内分泌学等方面的研究。目前常用的奶牛乳腺上皮细胞体外培养方法有酶消化法、组织块培养法、机械破碎法和乳汁分离法等方法。本研究主要综述了奶牛乳腺上皮细胞的体外培养方法、应用情况及发展趋势。

关键词: 乳腺, 奶牛乳腺上皮细胞, 细胞培养

Abstract: "Cell culture is the basis technique of cell biology;it is widely used in cell biology,molecular biology,and endocrinology.Currently in vitro the bovine mammary epithelial cells culture methods include enzyme digestion method,mammary tissue culture method,mechanical disruption and centrifugal method from milk based on their morphology.The application and the future trend of the bovine mammary epithelial cells culture in vitro methods were reviewed in this article. "

Key words: Mammary, Bovine mammary epithelial Cells, cell culture

中图分类号:

S823
Q813.1

李喜艳, 王加启, 魏宏阳, 卜登攀, 胡菡, 周凌云. 奶牛乳腺上皮细胞的体外培养方法及其应用概述[J]. 华北农学报, 2010, 25(S2): 31-35. doi: 10.7668/hbnxb.2010.S2.008.

LI Xi-yan, WANG Jia-qi, WEI Hong-yang, BU Deng-pan, HU Han, ZHOU Ling-yun. BovineMammary EpithelialCells CultureMethods in vitro and Its Application[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S2): 31-35. doi: 10.7668/hbnxb.2010.S2.008.

http://www.hbnxb.net/CN/Y2010/V25/IS2/31

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