摘要： 采用两阶段培养法研究不同培养基、激素的种类、浓度和各种营养成分对愈伤组织生长及紫草素形成的影响.结果表明,改良B培养基为适宜的营养生长培养基.细胞分裂素和生长素均为紫草素形成所必需的.6-BA 1.0 mg*L-1,IAA 0.1 mg*L-1为较佳配比.生长培养基中CaCl2浓度为20 mg*L-1,MgSO4为00 mg*L-1时,愈伤组织生长和紫草素形成均较佳.紫草素形成培养基中CuSO4为M9浓度的3倍时,紫草素产量较高.紫草素形成培养基中添加VB2及更高的FeSO4对紫草素产量的提高没有效果.研究过程中优化了紫草细胞培养形成紫草素的方法,为建立完整的组培生产紫草素体系提供了基础数据.
关键词:
硬紫草,
紫草素,
细胞培养
Abstract: Tw o-stage culture method w as used to study the ef fect of types and concentration of hormone, effect of types of culture medium, and nut rit ion on shikonin format ion. The result s show that both cytokinin and aux in are needed in shikonin formation. 6-BA 110 mg#L-1 and IAA 011 mg#L-1 is suitable in shikonin format ion medium. B5 medium is a better medium for cell growth. B5 w ith 250 mg#L-1 of CaCl2, 500 mg#L-1 of Mg SO4 are bet ter for both growth and shikonin format ion. M9 w ith three t imes of concentration of CuSO4 are bet ter choice for shikonin format ion. Addition of VB2 and more FeSO4 has no posit ive ef fect on shikonin format ion.
Key words:
L i thospermum erythroriz on,
Shikonin,
Cell culture
中图分类号:
严海燕, 曹日强. 紫草愈伤组织培养中紫草素形成的影响因素[J]. 华北农学报, 2002, 17(2): 116-120. doi: 10.3321/j.issn:1000-7091.2002.02.025.
YAN Haiyan, CAO Riqiang. Factors on Shikonin Formation in Cell Culture of Lithospermum erythrorizon[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(2): 116-120. doi: 10.3321/j.issn:1000-7091.2002.02.025.