摘要： 从细胞毒理学和免疫化学角度对磷酰胺除草剂APM对洋葱根尖细胞有丝分裂和微管功能的影响作了初步探讨,结果表明,经10 μmol/L APM处理16 h后,细胞出现多极分裂和不均等分裂现象,免疫化学检测表明,纺锤丝失去正常向两极辐射状排列的功能,而是凝聚成一团,故引起细胞的不正常分裂.
关键词:
磷酰胺除草剂,
多极分裂,
遗传毒理学,
免疫化学,
农业环境保护
Abstract: The effect of APM on cell division and microtubule funct ion at cy tolog ical and Immunbiochemical level were studied. The ex periment result s indicated that multipolar cell division, unequal cell division could be induced when root meristems were treated by 10 ..mol/ L APM for 16 h. Immun-biochemical test ing show ed that spindle f ibers were condensed and lost their regular arrangement tow ard the tw o poles. Abnormal cell division w as induced because the funct ion of spindle f ibers w as disturbed. It w as necessary to further study the genetic toxicity mechanism of APM on the cell division. It could be valuable in agriculture using phosphoric amide herbicide safely. Key words: Phosphoric amide herbicide; Multipolar cell division; Genet ic tox icity; Immun-biochemist ry; Agro-environmental protection。
中图分类号:
程罗根, 施新, 彭永康. 除草剂APM对洋葱细胞分裂和微管功能的影响[J]. 华北农学报, 2002, 17(2): 112-115. doi: 10.3321/j.issn:1000-7091.2002.02.024.
CHENG Luogen, SHI Xin, PENG Yongkang. The Effect of Phosphoric Amide Herbicide APM on Cell Division and Function of Microtubule in Allium Cepa[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(2): 112-115. doi: 10.3321/j.issn:1000-7091.2002.02.024.