摘要： 采用FILTRON中型盒式超滤系统，分别选用截留分子量为30，50，100及300 K 4种不同孔径的超滤膜，对NDV，AIV，EDS₇₆V，IBV及IBDV共5种病毒抗原液进行了浓缩试验研究。结果显示，这4种孔径的超滤膜均可用于上述5种病毒液的浓缩，其病毒的截留率可达99.9%～100%；采用各种病毒浓缩液、未浓缩病毒液及超滤液制成各种相应浓缩苗、未浓缩苗及超滤液乳剂免疫试验鸡。试验证明，浓缩苗明显优于未浓缩苗，在病毒浓缩过程中均未出现有效抗原成分的丢失，其抗原性也未发生变化。

关键词: 病毒浓缩, 疫苗, 免疫, 鸡

Abstract: By using the FILTRON medium-cassette ultra-filtration system, the viral antigens of newcastle disease virus (NDV), avian influenza virus (AIV), infectious bronchitis virus (IBV), infectious bursal disease virus (IBDV) and egg drop syndrome virus (EDS₇₆V) were concentrated with different pore size ultra-filtration membranes of 30, 50, 100 and 300 K respectively. The results showed that the above five viruses could be concentrated by 30, 50, 100 and 300 K molecular weight of ultra-filtration membranes, the virus recovery rate reached 99.90%-100%. The oil emulsion vaccines were made with concentrated virus fluid, virus fluid and ultra-filtrated fluid, and then were tested in chickens. The vaccination results revealed that concentrated virus vaccines were better than original virus vaccines. The effectiveness of virus fluid were not lost in the course of viral concentration and the virus antigenicity were not altered either.

Key words: Virus concentration, Vaccines, Vaccinat ion, Chicken

中图分类号: 

• S851.2