摘要： 引物的通用性研究对于降低引物开发的成本具有重要的意义。为此，本试验选取来源于小麦基因组的41对SSR引物，对其在早熟禾亚科不同属4种植物中的通用性进行了分析。所选取的41对引物在大麦、燕麦、小黑麦和三芒草4种供试植物中的扩增成功率分别为7.2%，82.9%，87.8%和85.4%，有些引物在特定植物中还会出现特异性条带，以上结果表明，从小麦基因组上开发的SSR引物，在早熟禾亚科不同种属植物间有较高的通用性。

关键词: 小麦基因组, SSR, 早熟禾亚科, 通用性

Abstract: General use analysis of primershas the vital significance to reduce their development cost. 41 pairs primers coming from the wheat gene set were selected in this experiment, and general use was carried on analysis among four species in different genus of Pooideae. In the barley, the oats, the triticale and three awn grass, the success ratio of these primers respectively was 73.2%, 82.9%, 87.8%and 85.4%, and some primers even still appeared specificity strip bands in particular plant, These results indicate that the SSR marker coming from the wheat gene set is highly transferable among species in different genus of Pooideae.

Key words: Wheat gene set, SSR, Pooideae, General use

中图分类号: 

• S512.1