小麦脂质转运蛋白cDNA结构及相应肽链构造特征的分析

doi:10.3321/j.issn:1000-7091.2007.02.001

华北农学报 ›› 2007, Vol. 22 ›› Issue (2): 1-5. doi: 10.3321/j.issn:1000-7091.2007.02.001

所属专题： 小麦；

• 论文 • 下一篇

小麦脂质转运蛋白cDNA结构及相应肽链构造特征的分析

孟成生^1,2, 李爱丽², 王省芬¹, 马峙英¹, 贾继增²

1. 河北农业大学农学院, 河北省作物种质资源重点实验室, 河北保定 071001;
2. 农业部作物种质资源与生物技术重点开放实验室, 中国农业科学院作物科学研究所, 北京 100081

收稿日期:2007-01-22 出版日期:2007-04-28
通讯作者: 马峙英(1958-)，男，河北新乐人，博士，教授，主要从事棉花遗传育种和分子生物学研究工作；贾继增(1945-)，男，河南偃师人，研究员，主要从事基于基因组学的种质资源研究和分子生物学研究。
作者简介:孟成生(1978-)，男，河北涉县人，硕士，主要从事小麦抗病机理和信号转导相关研究。
基金资助:
国家“973”计划项目(222092)

Sequence Analysis of Wheat Lipid Transfer Protein cDNA and the Structural Characteristics of Corresponding Peptide

MENG Cheng-sheng^1,2, LI Ai-li², WANG Xing-fen¹, MA Zhi-ying¹, JIA Ji-zeng²

1. Agricultural University of Hebei, Key Laboratory of Crop Germplasm Resources of Hebei, Baoding 071001, China;
2. Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2007-01-22 Published:2007-04-28

摘要/Abstract

摘要： 根据测序获得的1条261 bp cDNA片段，通过电子延伸、设计引物，从小麦Mardler/7^*百农3217(Pm2)的cDNA中扩增获得1条651 bp的cDNA片段，通过同源比对发现其包含小麦LTP1完整的编码序列。该片段包含基因的5′非翻译区42 bp，3′非翻译区261 bp，开放阅读框348 bp，编码115个氨基酸，预计蛋白的分子量为11.2 kD，等电点为9.46。此基因有8个位置保守的半胱氨酸(C)残基及25个氨基酸的信号肽，为典型的植物脂质转运蛋白基因。其基因序列数据库(GenBank)登录号为AY796184(基因)和AAV65513(蛋白)。通过疏/亲水性分析，发现肽链分子具有较大范围的疏水面。

关键词: 小麦, cDNA结构, 脂质转运蛋白

Abstract: One of the sequenced cDNA fragments was highly identified with LTP1. The EST was extended using CAP3 software in the lab local net. Based on the extended sequence, a pair of primers was designed in the two side regions of its predicted ORF. Using RT-PCR method, a cDNA fragment of 651 bp was isolated from Mardler/7^* BaiNong 3217. It was found that this cDNA contained the complete coding sequence of LTP1 of wheat including 42 bp of 5' untranslated region, 261 bp of 3' untranslated region, and an open reading frame (ORF) of 348 bp. The ORF region encoded a peptide of 115 deduced amino acid residues. The deduced molecular weight of protein was 11.2 kD and its isoelectric point was 9.46. This gene contained 8 cysteine residues that were conserved in amino acid sequence, and had a signal peptide with 25 amino acids. It was a typical plant LTP gene. The gene was accepted by GenBank, the accession numbers were AY796184 (gene) and AAV65513 (protein). The hydropathy profile revealed that the putative peptide chain contained several hydrophobic regions.

Key words: Wheat, cDNA structure, LTPs (Lipid transfer protein)

中图分类号:

S512.01

孟成生, 李爱丽, 王省芬, 马峙英, 贾继增. 小麦脂质转运蛋白cDNA结构及相应肽链构造特征的分析[J]. 华北农学报, 2007, 22(2): 1-5. doi: 10.3321/j.issn:1000-7091.2007.02.001.

MENG Cheng-sheng, LI Ai-li, WANG Xing-fen, MA Zhi-ying, JIA Ji-zeng. Sequence Analysis of Wheat Lipid Transfer Protein cDNA and the Structural Characteristics of Corresponding Peptide[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 1-5. doi: 10.3321/j.issn:1000-7091.2007.02.001.

http://www.hbnxb.net/CN/Y2007/V22/I2/1

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小麦脂质转运蛋白cDNA结构及相应肽链构造特征的分析

Sequence Analysis of Wheat Lipid Transfer Protein cDNA and the Structural Characteristics of Corresponding Peptide

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小麦脂质转运蛋白cDNA结构及相应肽链构造特征的分析

Sequence Analysis of Wheat Lipid Transfer Protein cDNA and the Structural Characteristics of Corresponding Peptide

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