摘要： 以2个高粱A₂类型雄性不育系及相应的保持系（A₂V4/B₂V4，A₂TX622/B₂TX622），2个恢复系（1383_2，晋粱5号）和3个组合（A₂V4×；1383_2，A₂V4×；晋粱5号，A₂TX622×；晋粱5号）的F₁，F₂群体为材料，采用SSR标记方法，分析A₂类型雄性不育的育性恢复基因。结果发现，只有标记Xtxp65在A₂TX622×；晋粱5号的F₂群体中与育性出现共分离现象，育性恢复基因与标记间距离为3.4cM，位于连锁群J上；同样只有标记Xtxp141在A₂V4×；晋粱5号的F₂群体中与育性出现共分离现象，该标记与育性恢复基因间的距离为13.4cM，位于连锁群G上；在A₂V4×；1383_2的F₂群体中，标记与育性未出现共分离现象。这说明，与这2个标记连锁的A₂CMS育性恢复基因是位于同一个恢复系（晋粱5号）中的2个独立的位点；在A₂恢复系材料中，至少有3个独立遗传的不同位点与A₂雄性不育性的恢复有关。

关键词: 高粱, CMS, A₂, SSR, 育性恢复基因

Abstract: In this study we investigated the locum of fertility restorer gene of A₂ cytoplasmic male sterility(CMS) in Sorghum with two cytoplasmic male sterile lines and maintainer lines(A₂V4/B₂V4,A₂TX622/B-2TX622),two restorers(1383-2,Jinliang 5) and F₁ and F₂ populations of three cross classifications(A₂V4×1383-2,A₂V4×Jinliang 5 and A₂TX622×Jinliang 5) by simple sequence repeat(SSR) technique.The results demonstrated that only one SSR marker,Xtxt65 was found to link with fertility restorer gene in F₂ population of A₂Tx622×Jinliang 5 and located in linkage groups(LG) J,the map distance between them was 3.4cM;only one SSR marker,Xtxt141 was found to link with fertility restorer gene in F₂ population of A₂V4×Jinliang 5 and located in LG G,the map distance between them was 13.4cM;no SSR marker was found to link with fertility restorer gene in F₂ population of A₂V4×1383-2.This showed that the fertility restorer genes linked with these two SSR marker were located two independent loci of one restore Jinliang 5,and at least three independent loci in restorers were associated with A₂cMS restoration.

Key words: Sorghum bicolor L.Meonch, CMS, A₂, SSR, Fertility restorer gene

中图分类号: 

• S514.03