摘要： 以马铃薯脱毒试管薯为试验材料,研究块茎休眠和块茎顶芽萌发生长过程中几种相关酶活性变化。结果表明,块茎贮藏过程中淀粉酶、淀粉磷酸化酶、过氧化物酶和多酚氧化酶活性的消长,与块茎形态上的休眠及萌发有密切关系。处于休眠期间的块茎内淀粉酶和淀粉磷酸化酶活性很低,随着休眠的解除及顶芽萌动活性逐渐增强,当顶芽开始生长时活性再次降低;过氧化物酶和多酚氧化酶在块茎休眠时活性较高,顶芽萌动后活性迅速下降;不同品种间这四种酶的变化趋势基本相同。

关键词: 马铃薯, 块茎, 休眠, 酶活性

Abstract: By analysis of the enzymes related to dormancy, it was found that activi t ies of amylase and ac t ivi ties of amylophosphorylase in dormant microtubers were much lower than these in dormant released tubers. It can be concluded hat energy was suppl ied by activating the amylase and amylophosphorylase during dormancy release. Peroxide activity was much higher in dormant microtubers than that in dormant released tubers.High active Peroxidase can reduce the concen tration of IAA. Dormancy and dormancy releasing could be regulated by peroxidase since high act iveperoxidase reduced concentration of IAA which affected tuber dormant releasing.The resul ts also sho wed polyphenol oxidase act ivity was higher in dormant microtubers than that in dormant released tubers. Polyphenol oxidase activity decreased lightly during dormancy breaking of potato microtubers. It inferred that polyphenol oxidase re gulated the respiration metabol ism during potato tuber dormancy as it was the enzyme related to respi ration.

Key words: Potato, Tuber, Dormancy, Activities of enzyme

中图分类号: 

• S532