摘要： 采用室内模拟盐、旱胁迫,结合田间实际测定的方法,对基因枪法获得的转BADH基因小麦多个株系的不同世代材料进行了耐盐、耐旱性鉴定。结果表明,在旱、盐胁迫条件下,转BADH基因小麦在种子萌发、幼苗生长、根系发育以及质膜保护等方面均比受体品种(对照)具有明显的优势;在田间生长条件下的转基因株系,其叶片蒸腾强度比对照明显降低,而离体叶片失水速率与对照没有明显差别。

关键词: 小麦, 转基因, BADH, 耐盐性, 耐旱性

Abstract: Assay for salt tolerance and drought resistance of transgenic wheat e xpressing betaine aldehyde dehydrogenase, obtained through part icle gun method,were conducted under simulated salt drought s tress condi tion. The resul ts in dicated that the transgenic wheat has many obvious advanta ges over its donor plants, such as the greater germinating ability under osmotic st ress up to-0. 3 Mpa, the more vigorous development of seedlings, especially the well developed roots under drought st ress condi tion, as well as the improved plasma membrane protection of excised leaf and the lower transpi ration rate under field condi tion. But the water los s rate for e xcised leaf was not obviously di fferent from its donor cont rol plants.

Key words: Wheat, Transgenic, Transgenic wheat, BADH, Drought Resistance, Salt Tolerance

中图分类号: 

• S512.1