摘要： 应用植物组织培养技术，研究了不同浓度的除草剂Basta对单子叶植物莜麦和双子叶植物荞麦种子萌发和生长的作用；应用高温高压灭菌法和过滤灭菌法对Basta进行了处理，比较了二者之间的灭菌效果。结果表明，当培养基中Basta的浓度超过4mg·L^-1时，可有效杀灭荞麦的种苗，而对莜麦种苗的有效作用浓度为6mg·L^-1以上。高温高压灭菌不会造成Basta的分解和杀灭效果的减弱。

关键词: 除草剂, 荞麦, 莜麦, 种胚, 组织培养

Abstract: Seeds of buckwheat and naked oats were sterilized and implanted on MS media in which concentration of herbicide Basta was 0,2,4,6,8 and 10mg·L^-1 respectively.After seeds of buckwheat germinated into seedlings,cotyledon gradually lost green and died on media supplemented with Basta 4mg·L^-1 or more.Germination and growth of naked oats seeds were inhibited when concentration of Basta was over 6mg·L^-1 in medium.High temperature and pressure method and filter method were effective to sterilization of Basta and there were no obvious differences between them in inhibition effect.

Key words: Herbicide, Buckwheat, Naked oats, Mature embryo, Tissue culture

中图分类号: 

• S17.03