华北农学报 ›› 2013, Vol. 28 ›› Issue (4): 98-104. doi: 10.3969/j.issn.1000-7091.2013.04.019

所属专题: 杂粮作物 盐碱胁迫 栽培生理

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外源多元醇对盐胁迫下荞麦种子萌发及幼苗生理特性的影响

杨洪兵   

  1. 青岛农业大学生命科学学院,山东省高校植物生物技术重点实验室, 山东青岛 266109
  • 收稿日期:2013-05-20 出版日期:2013-08-28
  • 作者简介:杨洪兵(1968- ),男,山东莒县人,副教授,博士,主要从事植物逆境生理与分子生物学研究
  • 基金资助:
    山东省自然科学基金项目(ZR2010CL019)

Effects of Exogenous Polyols on Seeds Germination and Seedlings Physiological Traits of Buckwheat under Salt Stress

YANG Hong-bing   

  1. Key Lab of Plant Biotechnology in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2013-05-20 Published:2013-08-28

摘要: 以盐敏感荞麦品种TQ-0808为试验材料,在100 mmol/L NaCl胁迫下添加不同浓度甘露醇和山梨醇处理,研究外源多元醇对荞麦种子萌发及幼苗生理特性的影响。结果表明,适当浓度的外源甘露醇和山梨醇处理能显著增加盐胁迫下荞麦种子的发芽率(Gr)、发芽指数(Gi)和活力指数(Vi),明显促进盐胁迫下荞麦幼苗生长及显著增加荞麦幼苗硝酸还原酶(NR)、超氧化物歧化酶(SOD)及抗坏血酸过氧化物酶(APX)活性,显著降低盐胁迫下荞麦幼苗的质膜透性,显著增加荞麦幼苗叶绿素含量和净光合速率。说明适当浓度甘露醇和山梨醇处理可以显著促进盐胁迫下荞麦种子萌发及幼苗生长,显著改善盐胁迫下荞麦幼苗的生理特性。外源甘露醇和山梨醇处理的最适浓度分别为0.8,0.6 mmol/L,且外源甘露醇处理的效果好于山梨醇处理。

关键词: 荞麦, 盐胁迫, 多元醇, 种子萌发, 生理特性

Abstract: Salt- sensitive buckwheat variety TQ-0808 was treated as experimental material.Under NaCl stress of 100 mmol /L and adding different concentrations of mannitol and sorbitol treatment to study the effects of exogenous polyols on seeds germination and seedlings physiological traits of buckwheat.The results showed that the appropriate concentrations of exogenous mannitol and sorbitol treatment could significantly increase the germination rate(Gr), germination index(Gi) and vigor index(Vi) of buckwheat seeds,and significantly promote the seedlings growth and increase the nitrate reductase(NR) activity,superoxide dismutase(SOD) activity and ascorbate peroxidase(APX) ac- tivity of buckwheat seedlings under salt stress,which could also significantly decrease the plasmalemma permeability of buckwheat seedlings under salt stress,and significantly increase the chlorophyll content and net photosynthetic rate of buckwheat seedlings.It indicated that the appropriate concentrations of mannitol or sorbitol treatment could significantly improve seeds germination and seedlings growth of buckwheat,and significantly improve the physiologi- cal traits of buckwheat seedlings.The optimal concentrations of exogenous mannitol and sorbitol treatment were 0.8, 0. 6 mmol /L respectively,and the effects of exogenous mannitol treatment were better than those of sorbitol did.

Key words: Buckwheat, Salt stress, Polyols, Seeds germination, Physiological traits

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引用本文

杨洪兵. 外源多元醇对盐胁迫下荞麦种子萌发及幼苗生理特性的影响[J]. 华北农学报, 2013, 28(4): 98-104. doi: 10.3969/j.issn.1000-7091.2013.04.019.

YANG Hong-bing. Effects of Exogenous Polyols on Seeds Germination and Seedlings Physiological Traits of Buckwheat under Salt Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 98-104. doi: 10.3969/j.issn.1000-7091.2013.04.019.