摘要： 以京欣一号、查理斯顿、巨人、井神小西瓜、黑崩筋5个品种苗龄6d的子叶为外植体，建立了西瓜品种无性繁殖系和高效的培养体系，在MS+BA5mg/L+IAA0.5mg/L培养基上，芽诱导率为46.6%～94%,转接到附加KT0.2mg/L的MS培养基上进行再生芽的伸长培养后，在MS+NAA0.5mg/L的根诱导培养基上的诱导率达96.2%.

关键词: 西瓜, 子叶, 组织培养, 植株再生

Abstract: An effective system for vegetative propagation and culture has been successfully set up by using cotyledons from five watermelon(Citrullus vulgaris) cultivars.High frequency shoot regeration(46 6—94%) was obtained from 6 day old cotyledons of cultivars cultured on medium containing MS+BA5mg/L+IAA0 5 mg/L.Buds elongated on MS medium containing KT 0 2mg/L.The MS medium with 0 1mg/L NAA was used for rooting and the rate of root induction was also above 80%.

Key words: Watermelon, Cotyledon, Tissue culture, Plant regeneration

中图分类号: 

• S651