摘要： 利用聚丙烯酰胺垂直平板凝胶电泳法，对普通小麦K、V型雄性不育系、保持系在不同发育时期的雄蕊、雌蕊、旗叶的过氧化物酶同工酶进行了系统研究。结果表明，K、V型不育系、保持系在不同发育时期的花药具有不同的酶谱。根据酶带数量和酶活性的变化，发现K、V型不育系之间在花粉败育时间上有较大差异。认为V型不育系败育的关键时期是“单核期”,而K型不育系为“二核期”,与细胞学观察结果相吻合。K、V型不育系、保持系之间在不同发育时期的雌蕊、旗叶上的同工酶未见明显差异

关键词: 普通小麦, 过氧化物酶, K型雄性不育系, V型雄性不育系, 保持系

Abstract: By means of vertical slab polyacrylamide gel electrophoresis,the isozyme zymograms of peroxidase were analyzed in the stamen,pistil and flag leaf from plants of Ae.kotschyi cytoplasmic male sterile line (K-CMS),and of Ae.ventricosa cytoplasamic male sterile line (V-CMS) as well as its maintenance line (B) at different developmental stages. The results showed that the isozyme zymograms of anthers of K-CMS,V-CMS and B were different at various developmental stages. According to the change of the isozyme bands and activity,it was found that the stage of pollen abortion was different between K-CMS and V-CMS. It was supposed that the key stage of pollen abortion was "single nucleus stage"for V-CMS, but "double nucleus stage"for K-CMS,This was in accordance with the observation of cytology.The isozyme zymograms of pistil and flag leaf of K-CMS,V-CMS,B showed no significantly different at different stages.

Key words: Common wheat, Peroxidase isozyme, K-type male sterile line, V-type male sterile line, Maintenance line

中图分类号: 

• S512.035