摘要： 本试验探索了甘薯过氧化物酶、酯酶、淀粉酶同工酶电泳的最佳条件, 利用这3种同工酶对本课题组保存的优势组合甘薯野生材料Y-6和栽培品种冀21-2及其由其杂交选育出的系列品种(品系)冀98、冀99、冀79、冀71、冀52进行深入的分析, 结果显示:优势组合Y-6与21-2的杂交后代中出现了杂种优势的特征酶谱类型——杂种型、偏亲本型等等, 各材料生产上的实际表现证实了同工酶分析的可靠性;表明:远缘组合Y-6与冀21-2属于强优势组合, 并且同工酶分析技术可以用于筛选鉴定甘薯优势组合及其杂交后代早期辅助选择, 加快育种进程。

关键词: 过氧化物酶, 酯酶, 淀粉酶, 远缘杂交, 优势组合

Abstract: This paper explored the best condition of the POD, EST, AMY, and analysised the wild sweetpotato Y-6, Ji21-2 and its five hybrid progenies including Ji98, Ji99, Ji79, Ji71, Ji52, its result showed that the reliability of the isozymes analysis in sweetpotato was validated from two aspects of existing zymogram types of the heterosis characteristics in the filial generation, such as the hybrid type, paternal or maternal type, combined with the performance of corresponding euhivar in the production. The cross combination wild sweetpotato Y-6 Ji21-2 belongs to the strong heterosis one, and the isozyme technique can be used to screen the strong combination and the early generation of the filial generation, and speeding up the breeding process.

Key words: Peroxidase, Esterase, Amylase, Distant hybridization, Superiority combination

中图分类号: 

• S531