摘要： 为提高绿色木霉Tr9701的发酵速率,实现其有效开发,对绿色木霉Tr9701液体培养过程中不同胞外酶活性和还原糖含量的动态变化进行了研究。结果表明,在25 ℃、110 r/min恒温振荡培养条件下,绿色木霉菌丝体生物量在第6~10天达到峰值;发酵液pH值在第6~10天发生转折逐渐上升,后基本保持平稳;胞外蛋白、胞外还原糖分别在第6,12天达到峰值,胞外愈创木酚氧化酶及多酚氧化酶活性以5 ~6 d为一个周期呈现曲线式的增强;其胞外淀粉酶、胞外羧甲基纤维素酶等酶的活性在培养前期有明显升高,且其液体发酵产羧甲基纤维素酶活性明显高于淀粉酶和蔗糖转化酶。为实现绿色木霉Tr9701规模发酵技术,应在其液体发酵过程中,依据菌丝体生物量、胞外酶和还原糖等动态变化确定其相应的发酵周期。

关键词: 绿色木霉, 液体发酵, 还原糖, 淀粉酶, 羧甲基纤维素酶, 愈创木酚氧化酶

Abstract: In order to improve the rate of fermentation and exploit Trichoderma Tr9701,the effect of liquid culture on different extracellular enzyme activity and the dynamic changes of reducing sugar content were studied.The results showed that at 25 ℃,110 r/min thermostatic culture conditions,biomass of green Trichoderma hyphae reached the peak in the 6-10 d;the pH value of the fermentation liquid occurs in the first 6-10 d turning gradually increased,then remained stable;extracellular proteins,extracellular sugar reached peak at 6,12 d,the secretion of guaiacol oxidase and polyphenol oxidase with a periodic curve of 5-6 d presents the enhancement curve type.The extracellular amylase,extracellular CMCase activity increased significantly in the early culture period,and the activity of extracellular CMCase was significantly higher than that of amylase and sucrose invertase in liquid fermentation.For realizing the scale fermentation of Trichoderma viride Tr9701,we should be determined the fermentation process according to the changes of extra-cellular enzyme activity and reducing sugar consistency during the Submerged culture of Tirchoderma viride.

Key words: Tirchoderma viride, Submerged culture, Reducing sugar, AmyLase, CMCase, Guaiccal oxidase

中图分类号: 

• S476+.8