摘要： 利用单因素筛选和均匀设计试验对拮抗性枯草芽孢杆菌B03发酵生产活菌体的液体培养基和发酵条件进行了研究,优化出了以经济、易得的玉米粉和豆饼粉为碳、氮源的最佳摇瓶发酵培养基,其配方为:玉米粉3.0%,豆饼粉6.0%,K2HPO4.3H2O 0.3%;最佳发酵条件为:种龄21~24 h,接种量5%,500 mL三角瓶装液量50 mL,初始pH值6.0,发酵温度35℃,摇床转速180 r/min,发酵周期60~66 h。利用优化后的培养基和发酵条件进行验证试验,获得了104.24×108~105.75×108cfu/mL的活菌体产量,较原始基础发酵培养基在常规培养条件下的菌体产量提高了96%以上。
关键词:
枯草芽孢杆菌,
菌体生产,
液体发酵,
均匀设计
Abstract: The medium and condition of liquid fermentation for biomass production of Bacillus subtilis B03 were optimized with mono-factor experiment and uniform design.The most suitable medium for living cell production was formulated with the ingredients:3.0% corn meal,6.0% soybean oil meal and 0.3% K2HPO4·3H2O.The optimized fermentation process was as follows:the seed culture was cultured for 21-24 h,the pH of production medium was adjusted to 6.0 before sterilization,the fermentation was carried out in 500 mL erlenmeyer flask with 50 mL production medium inoculated with 5% seed culture and incubated at 35℃,180 r/min for 60-66 h.The biomass density of 104.24×108-105.75×108 cfu/mL was obtained with optimized formula,which was over 96% higher than that produced by basic medium and initial fermentation process.
Key words:
Bacillus subtilis,
Biomass production,
Liquid fermentation,
Uniform design
中图分类号:
赵达, 刘伟成, 裘季燕, 刘霆, 傅俊范. 枯草芽孢杆菌B03液体发酵条件的优化[J]. 华北农学报, 2008, 23(2): 205-209. doi: 10.7668/hbnxb.2008.02.045.
ZHAO Da, LIU Wei-cheng, QIU Ji-yan, LIU Ting, FU Jun-fan. Optimization of the Liquid Fermentation Condition of Bacillus subtilis B03[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(2): 205-209. doi: 10.7668/hbnxb.2008.02.045.