摘要： 为确立的桃10 μL SRAP反应体系,以桃部分品种为试材,采用均匀设计,对SRAP-PCR反应体系中Taq DNA聚合酶、模板DNA、dNTPs、Mg2+、引物5个组分的浓度进行优化.结果表明,桃10 μL的SRAP反应体系的最佳组分包括2 U Taq DNA聚合酶、50 ng模板DNA、0.6 mmol/L dNTPs、0.25 μmol/L引物、2.0 μL 10×PCR Buffer+Mg2+.利用所确立的体系对其他部分桃种质进行扩增的结果清晰可靠,多态性好.并且利用随机挑选的SRAP引物和SSR引物分别区分鉴定8种桃种质,发现SRAP用5对可以将桃种质区分开,而SSR用13对才区分开,说明在桃品种鉴定上SRAP比SSR更省时方便,而且操作相对简单,适用性强.
关键词:
桃,
SRAP,
体系优化,
均匀设计,
SSR,
比较
Abstract: The Peach some varieties were used to adopt uniform design,the SRAP2PCR reaction system Taq DNA polymerase,the template DNA,dNTPs,Mg2 +,primers five components to optimize the concentration. The results showed that 10μL Peach SRAP reaction system of the best components,including 215μL 10 ×PCR Buffer + Mg2 + U Taq DNA polymerase,40 ng template DNA,012 mmol/ L dNTPs,012μmol/ L primer1Established by the use of the system to other parts of peach germplasm to amplify the results of clear and reliable,good polymorphism1And using random selection of the SRAP primer and SSR primer respectively distinction between identification of eight kinds of peach germplasm and found that SRAP can be used to separate peach germplasm,and the SSR was used to separate,that the peach variety iden2 tification on the SRAP in more than SSR Convenient,and relatively simple operation,the applicability.
Key words:
Peach,
SRAP,
System optimization,
Uniform design,
SSR,
Comparison
中图分类号:
郭瑞, 李晓燕, 王力荣, 王国山. 桃SRAP体系的优化及与SSR在桃品种鉴定上的比较[J]. 华北农学报, 2009, 24(4): 102-105. doi: 10.7668/hbnxb.2009.04.021.
GUO Rui, LI Xiao-yan, WANG Li-rong, WANG Guo-shan. Peach SRAP System Optimization and SSR Peach Varieties in the Comparative Identification[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 102-105. doi: 10.7668/hbnxb.2009.04.021.