摘要： 以产孢量为测定指标,通过单因子和正交试验对人参根部病害拮抗绿色木霉菌株Tv04-2固体发酵条件进行了筛选,获得了以玉米粉、麦麸等廉价农产品及其副产物为碳、氮源的最佳固体发酵培养基,其配方为:在250 mL三角瓶中装入20 g固体基质,其中玉米粉∶麦麸按1∶1比例混合,FeSO4含量0.1 g,水7 mL,接种量4 mL;最佳发酵条件为:25,20和28℃下依次发酵2 d。利用优化培养基及发酵条件进行发酵试验,可以获得高达4×109cfu/g的产孢量,为人参根部病害高拮抗活性木霉菌剂的生产和应用奠定了基础。
关键词:
绿色木霉,
固体发酵,
产孢量
Abstract: Optimal solid??state fermentat ion conditions were studied according to the sporulation quantity of Tricho??dermaviride isolate Tv04??2 by simple factor and orthogonal experiments.The most optimal medium was formulated as following: 250 mL erlenmeyer flask contained 20 g of equal quality of maize powder and wheat bran mixture, 0. 1 g FeSO4,7 mL water and 4 mL inoculum.The fermentat ion process was at a fluctuant temperature of 25, 20 and 28 for 2 days,respectively. Under this optimal medium and fermentation process, the spore concentrat ion of 4×109cfu/ g can be ob??tained, and which accelerated the production and applicat ion of Trichoderma viride strain with high antagonistic activity ofginseng root diseases.
Key words:
Trichoderma viride,
Solid fermentation,
Sporulat ion quantity
中图分类号:
刘时轮, 李勇, 傅俊范, 丁万隆, 方焕民. 绿色木霉菌株Tv04-2固体发酵条件研究[J]. 华北农学报, 2008, 23(S2): 244-247. doi: 10.7668/hbnxb.2008.S2.056.
LIU Shi-lun, LI Yong, FU Jun-fan, DING Wan-long, FANG Huan-min. Study on Fermentation Conditions of Antagonistic Trichoderma viride Tv04-2 Strain[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 244-247. doi: 10.7668/hbnxb.2008.S2.056.