玉米bZIP转录因子ZmbZIP26的分子特征、亚细胞定位及响应非生物胁迫的表达分析

doi:10.7668/hbnxb.20193892

摘要/Abstract

摘要：

bZIP转录因子广泛存在于植物中,在调节植物生长发育和非生物胁迫应答中起着重要作用。为探究bZIP转录因子在玉米干旱胁迫响应中的功能,在玉米苗期干旱胁迫处理5 d和复水3 d时,利用转录组测序技术对转录因子基因的表达变化进行分析,筛选到一个响应干旱和复水处理的bZIP转录因子(ZmbZIP26),共表达网络分析发现,ZmbZIP26处于网络调节的核心节点位置。ZmbZIP26基因含有558 bp的开放阅读框,编码185个氨基酸,属于亲水性蛋白。蛋白质进化树及保守序列分析发现,ZmbZIP26蛋白与高粱和芒草同源蛋白的同源性较高,而且在同一氨基酸位置上的保守基序相同。启动子顺式作用元件分析表明,ATG上游2 000 bp区域内含有干旱响应元件、激素响应元件和光响应元件等。qRT-PCR分析发现,ZmbZIP26是组成型表达基因,在幼茎、雌穗和根中高表达,且ZmbZIP26基因积极响应干旱、高温、高盐、氮胁迫及恢复正常的过程,可能在植物的抗逆过程中发挥着重要作用。亚细胞定位分析发现,ZmbZIP26属于核蛋白,定位在细胞核上。蛋白质互作预测发现,ZmbZIP26可能与锌指蛋白、丝氨酸蛋白、钙依赖性蛋白、谷胱甘肽转移蛋白等互作构建了一张调控网络,协同调控玉米生长发育和胁迫应答过程。

关键词: 玉米, bZIP转录因子, 干旱-复水处理, 非生物胁迫

Abstract:

bZIP transcription factors are widely found in plants and play an important role in regulating plant growth and development and abiotic stress response.In order to explore the functional role of bZIP transcription factor in maize drought stress response,transcriptome sequencing technology was used to analyze the expression changes of transcription factors in maize seedlings treated with drought stress for 5 days and rehydration for 3 days,and a bZIP transcription factor(ZmbZIP26)was screened from transcriptome data in response to drought and rewatering treatment.Co-expression network analysis revealed that ZmbZIP26 was at the core node of network regulation.The gene contained a 558 bp open reading frame encoding 185 amino acids,which was a hydrophilic protein.Phylogenetic tree and conserved sequence analysis showed that ZmbZIP26 protein had high homology with homologous proteins of sorghum and Miscanthus,and also had the same conserved motifs at the same amino acid positions.Cis-element analysis showed that the upstream 2 000 bp region of the ATG site contained drought response elements,hormone response elements and light response elements.qRT-PCR analysis showed that ZmbZIP26 was a constitutively expressed gene,which was highly expressed in young stems,female panicles and roots.ZmbZIP26 positively responded to drought,high temperature,high salt and nitrogen stress and the process of restoring,which might play an important role in the process of plant resistance.Subcellular localization analysis revealed that ZmbZIP26 was a nuclear protein localized in the nucleus.Protein interaction prediction showed that ZmbZIP26 might interact with zinc finger protein,serine protein,Ca-dependent protein and glutathione transfer protein to construct a regulatory network,which cooperatively regulated maize growth and development and stress response process.

Key words: Maize, bZIP transcription factor, Drought-restoring treatment, Abiotic stress

曹丽茹, 马晨晨, 庞芸芸, 叶飞宇, 王振华, 鲁晓民. 玉米bZIP转录因子ZmbZIP26的分子特征、亚细胞定位及响应非生物胁迫的表达分析[J]. 华北农学报, 2023, 38(4): 1-10. doi: 10.7668/hbnxb.20193892.

CAO Liru, MA Chenchen, PANG Yunyun, YE Feiyu, WANG Zhenhua, LU Xiaomin. Molecular Characteristics,Subcellular Localization and Expression Analysis of Maize bZIP Transcription Factor ZmbZIP26 in Response to Abiotic Stress[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(4): 1-10. doi: 10.7668/hbnxb.20193892.

http://www.hbnxb.net/CN/Y2023/V38/I4/1

图/表 10

参考文献 38

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蛋白质性质 Protein property	数值 Value
相对分子质量/ku Relative molecular mass	20.188 07
理论等电点 Theory PI value	10.50
不稳定系数 Instability index	71.82
亲水性值 Hydrophilic value	-0.443
α螺旋/% α-helix	73.51
β转角/% β-turn	0.54
无规则卷曲/% Random coil	20.54

蛋白质性质 Protein property	数值 Value
相对分子质量/ku Relative molecular mass	20.188 07
理论等电点 Theory PI value	10.50
不稳定系数 Instability index	71.82
亲水性值 Hydrophilic value	-0.443
α螺旋/% α-helix	73.51
β转角/% β-turn	0.54
无规则卷曲/% Random coil	20.54

元件 Element	序列 Sequence	位点 Site	来源 Source	功能注释 Function annotation
GTGGC-motif	GATTCTGTGGC	1 177	菠菜	光响应元件的一部分
CAAT-box	CAAAT	1 028	豌豆	启动子和增强子区域常见的顺式作用元件
Sp1	GGGCGG	985	水稻	光敏元件
GATA-motif	GATAGGA	315	拟南芥	光响应元件的一部分
CGTCA-motif	CGTCA	620	青稞	参与MeJA响应的顺式作用调节元件
G-Box	CACGTG	744	豌豆	参与光反应的顺式作用调节元件
Box 4	ATTAAT	444	欧芹	参与光响应的保守DNA模块的一部分
P-box	CCTTTTG	867	水稻	赤霉素响应元件
I-box	CGATAAGGCG	1 562	玉米	光响应元件的一部分
GC-motif	CCCCCG	1 835	玉米	参与缺氧特异性诱导的增强剂样元件
O2-site	GATGATGTGG	1 178	玉米	顺式作用调节元件参与玉米醇溶蛋白代谢调节
ABRE	ACGTG	618	拟南芥	参与脱落酸反应的顺式作用元件
G-box	CACGTG	744	拟南芥	参与光反应的顺式作用调节元件
A-box	CCGTCC	1 336	欧芹	顺式调节元件
TATA-box	TATA	130	拟南芥	转录起始点-30左右的核心启动子元件
MBS	CAACTG	553	拟南芥	MYB结合位点参与干旱诱导
GA-motif	ATAGATAA	626	拟南芥	光响应元件的一部分
TGACG-motif	TGACG	620	青稞	参与MeJA响应的顺式作用调节元件
TCA-element	CCATCTTTTT	1 742	烟草	参与水杨酸反应的顺式作用元件

元件 Element	序列 Sequence	位点 Site	来源 Source	功能注释 Function annotation
GTGGC-motif	GATTCTGTGGC	1 177	菠菜	光响应元件的一部分
CAAT-box	CAAAT	1 028	豌豆	启动子和增强子区域常见的顺式作用元件
Sp1	GGGCGG	985	水稻	光敏元件
GATA-motif	GATAGGA	315	拟南芥	光响应元件的一部分
CGTCA-motif	CGTCA	620	青稞	参与MeJA响应的顺式作用调节元件
G-Box	CACGTG	744	豌豆	参与光反应的顺式作用调节元件
Box 4	ATTAAT	444	欧芹	参与光响应的保守DNA模块的一部分
P-box	CCTTTTG	867	水稻	赤霉素响应元件
I-box	CGATAAGGCG	1 562	玉米	光响应元件的一部分
GC-motif	CCCCCG	1 835	玉米	参与缺氧特异性诱导的增强剂样元件
O2-site	GATGATGTGG	1 178	玉米	顺式作用调节元件参与玉米醇溶蛋白代谢调节
ABRE	ACGTG	618	拟南芥	参与脱落酸反应的顺式作用元件
G-box	CACGTG	744	拟南芥	参与光反应的顺式作用调节元件
A-box	CCGTCC	1 336	欧芹	顺式调节元件
TATA-box	TATA	130	拟南芥	转录起始点-30左右的核心启动子元件
MBS	CAACTG	553	拟南芥	MYB结合位点参与干旱诱导
GA-motif	ATAGATAA	626	拟南芥	光响应元件的一部分
TGACG-motif	TGACG	620	青稞	参与MeJA响应的顺式作用调节元件
TCA-element	CCATCTTTTT	1 742	烟草	参与水杨酸反应的顺式作用元件

蛋白质编号 Protein number	蛋白质描述 Protein description	氨基酸数目/个 Amino acid number	互作系数 Interaction coefficient
GRMZM2G024159	无注释	258	0.679
GRMZM2G159402	锌指蛋白8	231	0.679
GRMZM2G404316	无特性蛋白loc100277730	290	0.679
GRMZM2G008109	枯草杆菌样蛋白酶sbt3.5	568	0.678
GRMZM2G018943	翻译起始因子eIF-2B δ亚基	727	0.678
GRMZM2G109805	无特性蛋白loc100501360前体	538	0.678
GRMZM2G056612	CDPK相关激酶5	598	0.674
GRMZM2G471517	NPR1互作子	150	0.616
GRMZM2G409309	无注释	370	0.614
GRMZM2G428168	谷胱甘肽s-转移酶21	235	0.614

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