华北农学报 ›› 2021, Vol. 36 ›› Issue (3): 203-215. doi: 10.7668/hbnxb.20191958

所属专题: 土壤肥料

• 资源环境·植物保护 • 上一篇    下一篇

引入豆科作物的轮作模式对设施蔬菜土壤微生物群落组成的影响

刘蕾1, 徐梦2, 王凌1, 茹淑华1, 孙世友1, 郜静1, 李玭1, 张国印1, 马俊贤3   

  1. 1. 河北省农林科学院 农业资源环境研究所, 河北省肥料技术创新中心, 河北 石家庄 050051;
    2. 中国科学院 地理科学与资源研究所, 生态网络观测与模拟重点实验室, 北京 100101;
    3. 河北省市场监督管理局, 河北 石家庄 050091
  • 收稿日期:2020-12-27 出版日期:2021-06-28
  • 通讯作者: 张国印(1962-),男,山西陵川人,研究员,硕士,主要从事农业面源污染防控等研究;马俊贤(1970-),男,河北盐山人,高级经济师,主要从事农业资源管理研究。
  • 作者简介:刘蕾(1987-),女,河北石家庄人,助理研究员,博士,主要从事农业面源污染防控等研究。
  • 基金资助:
    国家重点研发计划课题(2017YFD0800404);河北省农林科学院博士资金项目

Effects of Crop Rotation with Legumes on the Composition of Microbial Community in Greenhouse Vegetable Soils

LIU Lei1, XU Meng2, WANG Ling1, RU Shuhua1, SUN Shiyou1, GAO Jing1, LI Pin1, ZHANG Guoyin1, MA Junxian3   

  1. 1. Institute of Agricultural Resources and Environment, Hebei Academy of Agriculture and Forestry Sciences, Hebei Fertilizer Technology Innovation Center, Shijiazhuang 050051, China;
    2. Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;
    3. Hebei Administration for Market Regulation, Shijiazhuang 050091, China
  • Received:2020-12-27 Published:2021-06-28

摘要: 基于豆科作物养分减投的轮作模式有助于在保障高产的基础上实现农业面源污染防控,为了探讨引入豆科蔬菜的轮作模式对土壤微生物群落的影响,采集定位试验3种轮作模式:西红柿-甜瓜、豆角-甜瓜、西红柿-豆角土壤样品,利用MiSeq高通量测序分析不同轮作模式下土壤微生物多样性、群落组成和结构差异,并结合土壤性质等环境因子探讨影响设施土壤微生物群落组成和结构的关键因素。结果表明,引入豆角的轮作模式显著影响土壤微生物的α-多样性,其中豆角-甜瓜模式显著降低了细菌Pielou指数,西红柿-豆角模式显著降低了真菌Chao指数。西红柿-豆角模式显著增加了土壤细菌中己科河菌门及真菌中担子菌门的相对丰度,分别是另2种轮作模式的2.3,4.1~4.8倍。在科水平上,西红柿-豆角模式显著增加了细菌亚硝化单胞菌科、Rokubacteriales、S085及真菌小不整球壳科、虫草菌科和丛赤壳科的相对丰度;豆角-甜瓜显著促进了细菌甾体杆菌科的生长;而西红柿-甜瓜显著提高了细菌Roseiflexaceae和生丝单胞菌科的相对丰度。CCA分析表明,地上部作物种类、pH值和NO3--N含量是影响土壤微生物群落组成和结构的关键环境因子,并且土壤细菌对环境因子改变的响应比真菌敏感。结果表明,相对于传统茄科-葫芦科轮作,引入豆科作物的轮作模式显著降低了细菌的均匀度和真菌的丰富度,并且改变了细菌和真菌的群落组成及结构,而且细菌对轮作模式改变的响应比真菌敏感。

关键词: 微生物群落, 设施土壤, 豆科, 轮作模式, 高通量测序

Abstract: The crop rotation system adjustment based on nutrient reduction of legumes is helpful to realize the prevention of agricultural non-point source pollution on the basis of ensuring high yield. To investigate the effect of different rotation systems on microbial communities, soil samples were collected from three rotation systems, i.e. tomato-melon, beans-melon and tomato-beans in location test. MiSeq pyrosequencing and soil properties analysis were conducted to analyze the differences in the diversity, composition and structure of microbial communities in soils under legume based rotations compared with those of traditional Solanaceae-Cucurbitaceae rotation and to determine the controlling factors of the variation in microbial communities. Results showed that the bacterial Pielou index and fungal Chao index were significantly reduced in soils of bean-melon soils and tomato-beans, respectively. The relative abundances of Rokubacteria (Bacterial phylum) and Basidiomycota (fungal phylum) were significantly increased by 2.3 and 4.1-4.8 times, respectively, in soils of tomato-beans compared to that in the other two systems. At family level, the relative abundances of Nitrosomonadaceae,Rokubacteriales and S085 (bacteria), and Plectosphaerellaceae,Cordycipitaceae and Nectriaceae (fungi) was significantly increased in soil of tomato-beans. The relative abundance of Steroidobacteraceae was significantly increased in soil of bean-melon, while the relative abundances of Roseiflexaceae and Hyphomonadaceae were promoted in soil of tomato-melon. Canonical correlation analysis (CCA) indicated that changes in the microbial community composition were strongly influenced by vegetable types, soil pH and NO3--N content. Moreover, we found that bacteria was more sensitive than fungi in response to changes in soil variables. Collectively, the present study showed that the adjustment of the rotation system by introducing legumes had significant impact on the soil microbial community by decreasing microbial diversity and altering community composition. Soil bacterial community responded more sensitively to the alteration of rotation system than fungi in greenhouse soils.

Key words: Microbial community, Greenhouse soil, Legume, Rotation, High-throughput pyrosequencing

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引用本文

刘蕾, 徐梦, 王凌, 茹淑华, 孙世友, 郜静, 李玭, 张国印, 马俊贤. 引入豆科作物的轮作模式对设施蔬菜土壤微生物群落组成的影响[J]. 华北农学报, 2021, 36(3): 203-215. doi: 10.7668/hbnxb.20191958.

LIU Lei, XU Meng, WANG Ling, RU Shuhua, SUN Shiyou, GAO Jing, LI Pin, ZHANG Guoyin, MA Junxian. Effects of Crop Rotation with Legumes on the Composition of Microbial Community in Greenhouse Vegetable Soils[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 203-215. doi: 10.7668/hbnxb.20191958.

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