干旱胁迫和复水处理后梭梭转录因子的转录组分析

doi:10.7668/hbnxb.20190513

摘要/Abstract

摘要： 为了探明梭梭应对干旱胁迫和水分刺激的分子调控机理，利用高通量测序技术对正常浇水（对照，CK）、干旱胁迫组（轻度干旱胁迫组，LWS；中度干旱胁迫组，MWS；重度干旱胁迫组，SWS）和复水组（RSWS）梭梭的转录组和转录因子表达谱进行分析。结果表明：数据经de novo拼接共获得74 641条非冗余Unigene，N50长度1 537 bp，对转录组数据比对、注释后共检测到56个转录因子家族、1 307个转录因子编码基因。与对照组相比，干旱胁迫组和复水组中的差异表达基因均以下调表达方式为主，其中，AP2-EREBP、MYB、bHLH、WRKY、NAC、ABI3VP1家族基因在各胁迫组中均表达且表达数较多。复水组中偏向通过增加AP2-EREBP、WRKY和NAC家族上调编码基因数量来实现梭梭幼苗对水分刺激的应答调节；而干旱组更偏向下调AP2-EREBP和bHLH的编码基因数量来实现幼苗对干旱胁迫的响应。干旱组和复水组测序结果的表达量（FPKM）与用荧光定量PCR法得到的基因表达量之间的相关性分别为0.947 8（P<0.01），0.967 2（P<0.01），结果证实了2种处理产生的差异表达基因数据的有效性。综合而言，AP2-EREBP、MYB和bHLH转录因子家族成员对梭梭干旱胁迫具有正负调控的作用。

关键词: 梭梭, 转录因子, 转录组测序, 干旱胁迫, 差异表达

Abstract: In order to elucidate the molecular regulation mechanism of H.ammodendron in response to drought stress and water stimulation,the transcriptome and expression profile of transcription factors of H.ammodendron in normal watering group(CK),drought stress groups(light water stress group (LWS),moderate water stress group(MWS),severe water stress group(SWS))and rehydration group after drought(RSWS)were systematically analyzed. The results showed that 74 641 non-redundancy unigenes with an average N50 length of 1 537 bp were obtained after being de novo assembled. And 1 307 transcription factor encoding genes belonging to 56 families were finally screened out by comparing and annotating the transcriptome data. Compared with the control group,the differentially expressed genes in the four treatment groups were mainly down-regulated,and the expression of AP2-EREBP,MYB,bHLH,WRKY,NAC,and ABI3VP1 transcription factors family were the most significant. In the rehydration group(RSWS vs SWS),the response to water stimulation of H.ammodendron seedlings was regulated by increasing the number of up-regulated AP2-EREBP,WRKY and NAC coding genes,however,in the drought-stress group(CK vs LWS,C vs MWS,CK vs SWS),the coding genes number of AP2-EREBP and bHLH was mainly down regulated. Nine transcription factor coding genes were randomly selected from the sequencing data. Real-time PCR was used to verify the validity of the sequencing data. The results showed that the correlation between the expression of FPKM in drought and rehydration groups and the gene expression obtained by fluorescence quantitative PCR was 0.947 8(P<0.01)and 0.967 2(P<0.01),respectively. In conclusion,the family members of AP2-EREBP,MYB and bHLH plays positive and negative regulation effect in response to the drought stress of H.ammodendron.

Key words: Haloxylon ammodendron, Transcription factor, Transcriptome, Drought stress, Differential expression

中图分类号:

S793.9

何江峰, 王力伟, 房永雨, 王蕴华, 王朝, 刘红葵. 干旱胁迫和复水处理后梭梭转录因子的转录组分析[J]. 华北农学报, 2020, 35(1): 36-43. doi: 10.7668/hbnxb.20190513.

HE Jiangfeng, WANG Liwei, FANG Yongyu, WANG Yunhua, WANG Chao, LIU Hongkui. Transcriptome Analysis on Transcription Factors of Haloxylon ammodendron under Drought Stress and Rehydration Treatment[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 36-43. doi: 10.7668/hbnxb.20190513.

http://www.hbnxb.net/CN/Y2020/V35/I1/36

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