摘要： BLEC1基因（C-type lectin-like receptor 1）编码C型凝集素样受体区域，与CD69家族的激活抗原相关，密切联系于禽类的先天性免疫功能。为了探究BLEC1基因在鸡中的遗传多样性以及不同鸡品种之间的抗病性差异，用苯酚和氯仿法提取26个不同鸡品种的DNA，对其BLEC1基因序列进行目标捕获测序，然后以NCBI上红色原鸡的BLEC1基因序列作为参考序列，序列号为NC_006103.4，通过MEGA 6软件对26个鸡品种的BLEC1基因序列进行序列比对分析，筛选出不同鸡种BLEC1基因序列上的SNPs位点和Indels位点以及对应氨基酸的变化，并根据基因序列的差异运用邻接法系统聚类分析构建进化树。在26个鸡品种中共检测到14个SNPs位点和12个Indels位点，其中有5个SNPs位点和3个Indels位点在外显子上，外显子上的3个Indels位点在鸡种中广泛存在，且Indel1存在于除黑狼山鸡以外的所有鸡种中，这些SNPs位点和Indels位点导致氨基酸发生极大改变，外显子中氨基酸有3个发生错义突变，其余为同义突变。邻接法系统聚类分析将26个鸡品种分为3大类，其中，安卡鸡、SPF-来航鸡和泰和乌骨鸡聚为一类；萧山鸡单独为一类；其余鸡种聚为一类。综上表明，BLEC1基因在我国地方鸡品种中有着丰富的遗传多样性，且与不同鸡种的先天性免疫功能和抗病性相关。

关键词: 鸡, BLEC1基因, SNPs, Indels, 进化树

Abstract: The BLEC1 gene (C-type lectin-like receptor 1)encodes a C-type lectin-like receptors that is associated with the activated antigen of the CD69 family and is closely linked to the innate immune function of poultry. In order to explore the genetic diversity of BLEC1 gene and different disease resistance of different chicken breeds. We used phenol and chloroform to extract the DNA of 26 different chicken breeds. Gallus gallus as a reference, the serial number was NC_006103.4, target sequence capture assay and MEGA 6 software was used to detect SNPs, Indels and amino acid sites in the BLEC1 gene of 26 chicken breeds. Finally, MEGA 6 software was used to construct phylogenetic trees. The results showed that 14 SNPs and 12 Indels loci were detected in 26 chicken breeds, of which 5 SNPs and 3 Indels loci were exon, 3 Indels sites on exons are widely present in chicken breeds. Indel1 was presented in all chicken breeds excepted Black Wolf Pheasant. These led to a great change in amino acids. In exons, 3 amino acids missense mutations, the rest of synonymous mutations. The neighbour joining cluster analysis was used to classify 26 chicken breeds into three groups, Anka chicken, SPF-Lai Hang chicken and Taihewugu chicken grouped together, Xiaoshan chicken alone for a categorize, the rest of the breeds were grouped together.All these suggested that BLEC1 gene was rich in genetic diversity in local chicken breeds in China and it was associated with innate immunity and disease resistance of different chicken.

Key words: Chickens, BLEC1 gene, SNPs, Indels, Phylogenetic tree

中图分类号: 

• Q78

• S831