玉米雄穗发育早期耐旱相关转录因子的发掘

doi:10.7668/hbnxb.2018.04.001

摘要/Abstract

摘要： 为了发掘更多与玉米雄穗生长发育早期耐旱性相关的转录因子基因，选用耐旱型玉米自交系（DT）铁7922、X178，以及干旱敏感型自交系（DS）吉81162和丹340为试验材料。使用盆栽方法进行育苗，并在一致且适宜的肥水条件下培养至雄穗发育前期，通过人工模拟田间干旱胁迫及正常灌溉的方法对材料进行处理。采用RNA-Seq技术检测玉米雄穗发育早期在受到干旱胁迫后相对于正常灌溉处理而言，耐旱相关转录因子家族基因在转录水平上表达量的变化。结果共发现287个转录因子基因在受到干旱胁迫后相对于正常灌溉呈现差异表达。对差异表达基因进行功能富集和注释结果发现，差异表达基因中具有耐旱调控功能的转录因子基因34个。其中在2个耐旱型材料中共有且表达模式一致的基因6个；在2个干旱敏感型材料中共有且表达模式一致的基因3个；在4个材料中均呈显著差异表达的转录因子基因1个。研究结果为玉米雄穗发育早期耐旱功能转录因子基因发掘和耐旱分子育种提供了基因基础。

关键词: 玉米雄穗, 转录因子, 耐旱性

Abstract: To explore more drought-related transcription factors genes in early growth and development of tassels, two drought-tolerant (DT) Tie 7922, X178 and two sensitive maize inbred lines (DS) Ji 81162, Dan 340 were used. The potted planting method was used to cultivate the seedlings, and the materials were treated by artificial simulated field drought stress and normal irrigation under the same and suitable water conditions. RNA-Seq technique was used to detect the changes in transcriptional level of the gene in drought related transcription factor family in the early stage of maize ear development compared with the normal irrigation treatment. The results showed that 287 genes were differently expressed under drought stress, and 34 of them are related to drought tolerance regulation revealed by GO enrichment and annotation, including 6 commons in two drought-tolerant line, 3 commons in two sensitive line, and all with uniform expression pattern, and 1 detected in four inbreds. The results provided the basic knowledge for drought tolerant gene identifition and molecular breeding of maize.

Key words: Maize tassel, Transcription factors, Drought tolerance

中图分类号:

Q78
S513.03

吴永波, 郝转芳, 王楠, 宋洁, 周跃恒, 柳波娟, 朱汉勇, 邸宏, 王振华, 李新海. 玉米雄穗发育早期耐旱相关转录因子的发掘[J]. 华北农学报, 2018, 33(4): 1-8. doi: 10.7668/hbnxb.2018.04.001.

WU Yongbo, HAO Zhuanfang, WANG Nan, SONG Jie, ZHOU Yueheng, LIU Bojuan, ZHU Hanyong, DI Hong, WANG Zhenhua, LI Xinhai. The Identification of Drought Tolerance-related Transcription Factors in Early Developing Tassel of Maize[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 1-8. doi: 10.7668/hbnxb.2018.04.001.

http://www.hbnxb.net/CN/Y2018/V33/I4/1

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