芸豆盐碱响应NAC转录因子的分子特性分析

doi:10.7668/hbnxb.20192387

摘要/Abstract

摘要： 为进一步揭示芸豆NAC基因在参与调控植物生长发育及应对非生物胁迫反应中的作用，以芸豆耐盐碱品种HYD为试验材料，利用Illumina HiSeq技术，构建了NaHCO₃处理和NaCl处理下的芸豆叶片组织转录组。从转录组数据中共筛选出8个盐碱相关NAC转录因子，并对其进行理化性质分析、系统进化发育分析、磷酸化位点预测、蛋白质二级结构预测、启动子元件分析、基因结构分析、染色体定位分析和表达量分析。结果表明：8个芸豆盐碱响应NAC基因分子量在23 676.36~44 354.33 ku，蛋白编码203~394个氨基酸，等电点在4.74~8.86，其中3个基因编码酸性蛋白，8个蛋白被分为4个亚族（a~d），分列在8个染色体上，包含3个膜结合蛋白，8个蛋白中丝氨酸数量最多，有10~22个，无规则卷曲结构占比最大，在68.0%~84.5%，8个蛋白全部定位在细胞核上。在NaHCO₃处理下，4个基因为上调表达（log₂FC>2），2个为下调表达（log₂FC<-2），NaCl处理下，1个为上调表达（log₂FC>2），4个为下调表达（log₂FC<-2），有3个基因同时响应NaHCO₃和NaCl。在8个基因启动子中共发现10个启动子元件，同时每个基因都包含3~7个元件。表明这些基因都可能参与芸豆的逆境应答。

关键词: 芸豆, NAC转录因子, 生物信息学, 盐碱胁迫

Abstract: In order to further reveal the role of kidney common bean NAC gene in regulating plant growth and development and in response to abiotic stress, this research was used the salt-tolerant common bean variety HYD as the test material and used the Illumina HiSeq technology, the transcriptome of common bean leaf tissue under the NaHCO₃ and NaCl treatments was constructed. A total of 8 saline-alkaline related NAC transcription factors were screened from the transcriptomic data, and carried out analysis of physicochemical property, systematic evolution, phosphorylation site prediction, protein secondary structure prediction, promoter element analysis, gene structure analysis, chromosomal location analysis and expression analysis. The result showed:The molecular weight of 8 common bean saline-alkaline response NAC genes was 23 676.36-44 354.33 ku, and the protein codes were 203-394 amino acids. The isoelectric point was between 4.74 and 8.86, of which 3 genes encoded acidic proteins, and 8 genes were divided into 4 subfamilies (a-d), positioning in 8 chromosomes, including 3 membrane-bound proteins, the number of serine was the largest in 8 proteins, there were 10-22, and the random coil structure accounts for the largest proportion, 68.0% to 84.5%. All 8 proteins were located in the nucleus. Under NaHCO₃ treatment, 4 genes were up-regulated expression (log₂FC>2), 2 genes were down-regulated expression (log₂FC<-2), Under NaCl treatment, 1 gene was up-regulated expression (log₂FC>2), 4 genes were down-regulated expression (log₂FC<-2), 3 genes respond to both NaHCO₃ and NaCl. A total of 10 promoter elements were found in 8 gene promoters, and each gene contained 3-7 elements. It indicated that these genes might be involved in the stress response of common beans.

Key words: Common bean, NAC transcription factor, Bioinformatics, Saline-alkali stress

中图分类号:

S643.1

翟玲侠, 于崧, 侯玉龙, 秦猛, 朱雪天, 王小琴, 于立河. 芸豆盐碱响应NAC转录因子的分子特性分析[J]. 华北农学报, 2021, 36(6): 19-27. doi: 10.7668/hbnxb.20192387.

ZHAI Lingxia, YU Song, HOU Yulong, QIN Meng, ZHU Xuetian, WANG Xiaoqin, YU Lihe. Molecular Characterization of Saline-Alkaline Responsive NAC Transcription Factors in Common Bean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 19-27. doi: 10.7668/hbnxb.20192387.

http://www.hbnxb.net/CN/Y2021/V36/I6/19

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