卵型家族蛋白转录因子AtOFP8的抗旱性功能分析

doi:10.7668/hbnxb.20190669

摘要/Abstract

摘要： 为了探究拟南芥转录因子AtOFP8（At5g19650）的功能以及明确其作用机制，以Col、Atofp8-1突变体和35S：HA-AtOFP8过表达拟南芥为试验材料，采用盆栽模拟自然界干旱的方法，连续控水15 d，通过对试验材料进行表型观察分析，同时测定试验材料在干旱胁迫下的相关生理指标以及植株体内干旱应答基因做出的反应。结果表明：随着干旱胁迫的加剧，35S：HA-AtOFP8植株的种子萌发率和绿叶率均最高，Atofp8-1突变体则最低；干旱胁迫15 d时，Atofp8-1突变体、Col和35S：HA-AtOFP8拟南芥叶片中MDA的含量分别较对照升高了229.8%，130.4%和71.1%；随着干旱胁迫时间的增加，Col、Atofp8-1突变体和35S：HA-AtOFP8植株叶片中抗氧化酶活性、脯氨酸和可溶性蛋白含量均有不同程度的增加，但35S：HA-AtOFP8植株叶片中抗氧化酶活性、脯氨酸和可溶性蛋白含量始终高于Col和Atofp8-1突变体；实时定量PCR的分析结果显示，35S：HA-AtOFP8植株体内干旱应答基因（AtADH1、AtRD26、AtRDUF2、AtERD7 ）的表达量均高于Atofp8-1突变体。综合说明拟南芥转录因子AtOFP8赋予了拟南芥更高的耐旱性。

关键词: 卵型家族蛋白, 转录因子, AtOFP8, 抗旱性

Abstract: To explore the function of the Arabidopsis thaliana transcription factor AtOFP8 (At5g19650) and to clarify its mechanism of action, the Col wild type Arabidopsis thaliana, Atofp8-1 mutant and 35S:HA-AtOFP8 transgenic plants were used as test materials, method of simulating drought in nature using potted plants, continuous water control 15 d, through the phenotypic observation and analysis of the experimental materials, the relevant physiological indexes of the experimental materials under drought stress and the responses of the drought response genes in the plants were simultaneously determined. Results showed:with the increase of drought stress, 35S:HA-AtOFP8 overexpression had the highest seed germination rate and green leaf rate, while Atofp8-1 mutant had the lowest; When drought stress was 15 days, the content of MDA in the leaves of Atofp8-1 mutant, Col wild type and 35S:HA-AtOFP8 overexpression was increased by 229.8%, 130.4% and 71.1%, respectively. As the drought stress time increases, the activities of antioxidant enzymes, proline and soluble protein in leaves of Col, Atofp8-1 deletion mutant and 35S:HA-AtOFP8 overexpressing plants increased to varying degrees, however, the antioxidant enzyme activity, proline and soluble protein content in leaves of 35S:HA-AtOFP8 overexpression were consistently higher than Col wild type and Atofp8-1 mutant. Analysis of Real-time PCR showed that the expression of drought response genes (AtADH1,AtRD26,AtRDUF2,AtERD7) in 35S:HA-AtOFP8 overexpression was higher than that of Atofp8-1 mutant. This indicates that Arabidopsis thaliana transcription factor AtOFP8 confers higher drought tolerance.

Key words: Ovate family proteins, Transcription factor, AtOFP8, Drought resistance

中图分类号:

刘守银, 唐尧, 韩佳良, 常缨. 卵型家族蛋白转录因子AtOFP8的抗旱性功能分析[J]. 华北农学报, 2020, 35(5): 98-106. doi: 10.7668/hbnxb.20190669.

LIU Shouyin, TANG Yao, HAN Jialiang, CHANG Ying. Drought Resistance Analysis of Ovate Family Proteins Transcription Factor AtOFP8[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 98-106. doi: 10.7668/hbnxb.20190669.

http://www.hbnxb.net/CN/Y2020/V35/I5/98

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