摘要： 为了进一步研究TaEF-1α基因在小麦响应干旱胁迫中的作用，首先在正常灌水和干旱胁迫2个处理下对TaEF-1α基因在转录水平上的表达量进行了验证，并利用VIGS（Virus induced gene silencing）技术将TaEF-1α基因进行沉默，对其功能进行研究。结果表明，TaEF-1α基因在mRNA水平上的表达量与其蛋白水平上的变化趋势一致，均受干旱诱导上调表达。与正常灌水且未进行VIGS沉默处理的植株相比，干旱处理且未进行VIGS沉默处理的植株中TaEF-1α基因表达量极显著升高，其叶片相对含水量也极显著降低、相对电导率和丙二醛（MDA）含量则极显著升高；干旱条件下，与未进行VIGS沉默的对照植株相比，VIGS沉默后的植株中TaEF-1α基因表达量极显著下降，其叶片相对含水量也极显著降低、相对电导率和丙二醛（MDA）含量则极显著升高，植株表现出叶片下垂、对干旱胁迫敏感的表型。综合干旱处理和VIGS沉默TaEF-1α基因后小麦植株的表型和生理指标变化，说明干旱胁迫诱导小麦植株中TaEF-1α基因上调表达，敲低TaEF-1α基因表达后 小麦抗旱性显著下降，因此，TaEF-1α基因在小麦响应干旱胁迫中可能发挥了重要作用。

关键词: 小麦, 干旱胁迫, TaEF-1α基因, VIGS技术

Abstract: The expression level of the protein product of TaEF-1α gene was up-regulated under drought stress condition in one of our previous study using iTRAQ technology. To further study the function of TaEF-1α gene in response to drought stress in wheat,we quantified the expression of TaEF-1α gene at the transcriptional level gene under normal and drought stress conditions, the expression level of TaEF-1α gene was knock down by using virus induced gene silencing(VIGS) technique to study its function. The results showed that the expression of TaEF-1α gene was up-regulated by drought stress. Under drought stress condition, the expression level of TaEF-1α gene and the relative water content of leaves decreased significantly and the relative conductivity and malondialdehyde(MDA) content increased significantly in knock-down-plants than wild type plants. The changes in phenotypes and physiological indices of wheat plants after drought stress and VIGS silencing TaEF-1α gene indicated that the drought-induced TaEF-1α gene expression in wheat plants was up-regulated, and the TaEF-1α gene knock-down-plants also exhibited leaf drooping earlier and more sensitive to drought stress than wild type plants. Taken together, TaEF-1α gene may play a crucial role in response to drought stress in wheat.

Key words: Wheat, Drought stress, TaEF-1 &alpha, gene, VIGS technique

中图分类号: 

• Q78

• S512.03