菘蓝<i>IiCYP79B2</i>基因的克隆与表达分析

doi:10.7668/hbnxb.201751719

摘要/Abstract

摘要： 为了探究菘蓝IiCYP79B2基因的结构、特性和功能，对菘蓝IiCYP79B2基因进行了克隆，并进一步开展了生物信息学分析和不同条件下的表达模式研究。相关研究结果显示：IiCYP79B2基因全长1 827 bp，包含1个内含子，ORF全长1 629 bp，编码542个氨基酸。其编码的蛋白含有2个跨膜结构域，无信号肽，定位于叶绿体类囊体膜上，属于主要由无规则卷曲和α-螺旋构成的亲水性蛋白，与白芥的亲缘关系最近。qRT-PCR分析结果表明：IiCYP79B2基因在菘蓝各组织中的表达量由高到低依次为叶 > 茎 > 果 > 花 > 根，在不同发育时期的表达量依次为营养生长期 > 花期 > 幼苗期 > 萌芽期；此外，该基因的表达还显著响应茉莉酸甲酯（MJ）和葡萄糖（Glu）信号的诱导，受到水杨酸（SA）和低温（Cold）胁迫的抑制。相关研究结果为进一步探讨IiCYP79B2基因的功能提供了有效的试验依据。

关键词: 菘蓝, IiCYP79B2, 基因克隆, 表达分析

Abstract: In order to explore the structure, characteristics and functions of IiCYP79B2 gene in Isatis indigotica, the IiCYP79B2 gene was cloned and the bioinformatics analysis and expression patterns under different conditions were carried out. The results showed that the full length of the gene was 1 827 bp with an intron, and its ORF was 1 629 bp, encoding 542 amino acids. IiCYP79B2 protein contained two transmembrane domains without signal peptide. The protein was localized in the chloroplast thylakoid membrane and mainly consisted of random coils and α-helix structures, having the closest relationship with Sinapis alba. The qRT-PCR analysis showed that the expression levels of IiCYP79B2 in different tissues were leaf > stem > fruit > flower > root, and the expression levels at different development stages were vegetative growth > flowering > seedling > germination. Moreover, IiCYP79B2 could be induced significantly by MJ and Glu, and repressed by SA and cold. The obtained results provided some experimental evidences for further studying the functions of IiCYP79B2 gene.

Key words: Isatis indigotica Fort., IiCYP79B2, Gene cloning, Expression analysis

中图分类号:

S567.03
Q78

胡向阳, 屈新运, 吴格格, 秦苗苗, 刘蕊, 高天娥, 李焘. 菘蓝IiCYP79B2基因的克隆与表达分析[J]. 华北农学报, 2019, 34(5): 23-29. doi: 10.7668/hbnxb.201751719.

HU Xiangyang, QU Xinyun, WU Gege, QIN Miaomiao, LIU Rui, GAO Tian, LI Tao. Cloning and Expression Analysis of IiCYP79B2 from Isatis indigotica Fort.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 23-29. doi: 10.7668/hbnxb.201751719.

http://www.hbnxb.net/CN/Y2019/V34/I5/23

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菘蓝IiCYP79B2基因的克隆与表达分析

Cloning and Expression Analysis of IiCYP79B2 from Isatis indigotica Fort.

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菘蓝IiCYP79B2基因的克隆与表达分析

Cloning and Expression Analysis of IiCYP79B2 from Isatis indigotica Fort.

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