苹果<em>MdSBP20</em>基因抗非生物胁迫的研究

doi:10.7668/hbnxb.2016.03.008

华北农学报 ›› 2016, Vol. 31 ›› Issue (3): 51-57. doi: 10.7668/hbnxb.2016.03.008

所属专题： 苹果；生物技术；果树专题；

苹果MdSBP20基因抗非生物胁迫的研究

沙婷¹, 侯鸿敏¹, 孟祥娜¹, 柏素花¹, 周爱琴¹, 沙广利², 戴洪义¹, 祝军¹

1. 青岛农业大学园艺学院, 山东青岛 266109;
2. 青岛市农业科学研究院, 山东青岛 266100

收稿日期:2016-03-06 出版日期:2016-06-28
作者简介:沙婷(1988-),女,内蒙古牙克石人,硕士,主要从事果树分子生物学研究。
基金资助:
国家苹果产业技术体系项目(CARS280107);"十二五"国家科技支撑计划项目(2013BAD02B01-2);中央财政农业技术推广项目;山东省良种工程项目;山东省优秀中青年科学家奖励基金项目(BS2014SW027);青岛市科技计划项目(14-2-3-38-nsh);青岛农业大学高层次人才科研基金项目(663/1114338)

Study on Abiotic Stress of MdSBP20 Gene from Malus

SHA Ting¹, HOU Hongmin¹, MENG Xiangna¹, BAI Suhua¹, ZHOU Aiqin¹, SHA Guangli², DAI Hongyi¹, ZHU Jun¹

1. College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China;
2. Qingdao Academy of Agricultural Sciences, Qingdao 266100, China

Received:2016-03-06 Published:2016-06-28

摘要/Abstract

摘要： 为获得苹果砧木逆境胁迫相关转录因子,以QZ1(平邑甜茶(Malus hupehensis Rehd.)×柱型苹果株系CO(Malus domestica Borkh.)和M26为试材,利用同源克隆法克隆得到苹果SBP基因cDNA的全长序列,命名为MdSBP20。对扩增得到的cDNA序列进行生物信息学分析,结果表明,该序列全长1362bp,包含完整开放阅读框1362bp,编码454个氨基酸,具有明显的SBP结构域,包含2个锌指结构Zn-1、Zn-2和双向核定位信号区NLS。系统进化树分析,结果表明MdSBP20与白梨(XM_009339726.1)和苹果(XM_008376435.1)亲缘关系较近。实时荧光定量分析结果表明,MdSBP20基因在低温、干旱、盐害中发挥一定功能,推测QZ1的耐寒性、耐旱性及耐盐性均强于M26。研究表明,MdSBP20基因在苹果响应非生物逆境胁迫中具有重要调控作用。

关键词: 苹果, 非生物胁迫, MdSBP20基因, 基因克隆, 表达分析

Abstract: To obtain the transcription factor related to stress conditions in apple rootstock,an apple SBP gene with the full-length cDNA was cloned in Qingzhen 1(apomictic crabapple Pingyitiancha( Malus hupehensis Rehd.)× columnar apple strain CO(Malus × domestica Borkh.) and M26 via homology-based cloning method.The gene cloned was named as MdSBP20 .Bioinformatics analysis to the cDNA showed that the full-length cDNA was 1 362 bp,it's open reading frame possessed 1 362 bp,and encoded 454 amino acids,which had obviously SBP -domain and two zinc finger structures(Zn-1,Zn-2) and the bothway nuclear location signal(NLS).Phylogenetic analysis suggested that MdSBP20 gene had the closer relationship with Pyrus bretschneideri (XM_009339726.1)and Malus domestica (XM_008376435.1).Real-time PCR analysis showed that MdSBP20 gene played a role in response to low temperature,drought,and salt tolerance.It was inferred that QZ1 might has higher resistance than M26 no matter in stress of low temperature,drought,and salt.Based on the results in this experiment, MdSBP20 gene had the significantly effects in apple rootstock response to abiotic stress.

Key words: Apple(Malus ), Abiotic stress, MdSBP20 gene, Gene clone, Expression analysis

中图分类号:

Q78
S661.1

沙婷, 侯鸿敏, 孟祥娜, 柏素花, 周爱琴, 沙广利, 戴洪义, 祝军. 苹果MdSBP20基因抗非生物胁迫的研究[J]. 华北农学报, 2016, 31(3): 51-57. doi: 10.7668/hbnxb.2016.03.008.

SHA Ting, HOU Hongmin, MENG Xiangna, BAI Suhua, ZHOU Aiqin, SHA Guangli, DAI Hongyi, ZHU Jun. Study on Abiotic Stress of MdSBP20 Gene from Malus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 51-57. doi: 10.7668/hbnxb.2016.03.008.

http://www.hbnxb.net/CN/Y2016/V31/I3/51

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