节水灌溉对冬小麦田土壤微生物特性、土壤酶活性和养分的调控研究

doi:10.7668/hbnxb.2016.01.036

摘要/Abstract

摘要： 为了探讨节水灌溉下土壤微生物特性和土壤酶活性的变化特征及其与土壤养分之间的关系,在华北平原旋耕条件下,研究了不同灌溉处理(常规灌溉W1、节水灌溉W2和无灌溉W3,冬小麦全生育期总灌溉量分别为150,75,0 mm)对冬小麦田土壤微生物量氮、基础呼吸、土壤酶活性和土壤养分的影响。结果表明:减少灌溉量可显著降低土壤微生物量氮和土壤基础呼吸。随着灌溉量的减少,β-葡萄糖苷酶和多酚氧化酶活性随之降低;而W3可显著提高脲酶活性。土壤含水量随灌溉量的减少而降低。水分胁迫可显著提高土壤有机碳和硝态氮含量,但有降低铵态氮含量的趋势。相关性分析可知,土壤微生物量氮与土壤铵态氮含量显著正相关。β-葡萄糖苷酶和多酚氧化酶活性均与土壤含水量和铵态氮含量显著正相关,β-葡萄糖苷酶活性与有机碳含量显著负相关;脲酶活性与有机碳和硝态氮含量显著正相关,而与土壤含水量显著负相关。可见土壤微生物特性和土壤酶活性受灌溉处理调控,并且与土壤C、N养分的循环转化关系密切。

关键词: 灌溉, 土壤酶, 微生物量氮, 微生物特性

Abstract: In order to investigated the dynamic change of soil microbial characteristics and soil enzyme activities and the correlation among soil microbial characteristics,soil enzyme activities and soil nutrient,a field experiment was conducted to study the effects of different irrigation methods(conventional irrigation,saving-irrigation and no irrigation,150,75,0 mm of irrigation rate during winter wheat growing season respectively,named by W1,W2 and W3 respectively) on soil microbial biomass nitrogen,soil basal respiration,soil enzyme activities and soil nutrient in the winter wheat field.These results indicated that the reduction of irrigation rate decreased significantly soil microbial biomass nitrogen and soil basal respiration.With the decrease of irrigation rate,β-glucosidase and polyphenol oxidase activities were decreased,but W3 increased markedly urease activity.Soil water content was decreased as the irrigation rate decreasing.Water stress enhanced significantly soil organic carbon and nitrate nitrogen content,however,ammonium nitrogen content had a decreased trend under water stress.Soil microbial biomass nitrogen was significantly positively correlated with soil ammonium nitrogen content.β-glucosidase and polyphenol oxidase activities were significantly positively correlated with soil water and ammonium nitrogen content,and there was a negative significant correlation between β-glucosidase activity and organic carbon content.Urease activity was significantly positively correlated with organic carbon and nitrate nitrogen content,but was significantly negatively correlated with soil water content. The results suggested that soil microbial characteristics and soil enzyme activities were regulated by irrigation and they were correlated with recycling and transformation of C and N.

Key words: Irrigation, Soil enzyme, Microbial biomass nitrogen, Microbial characteristic

中图分类号:

S152

叶德练, 齐瑞娟, 张明才, 李召虎. 节水灌溉对冬小麦田土壤微生物特性、土壤酶活性和养分的调控研究[J]. 华北农学报, 2016, 31(1): 224-231. doi: 10.7668/hbnxb.2016.01.036.

YE Delian, QI Ruijuan, ZHANG Mingcai, LI Zhaohu. Study of Saving-irrigation Regulated the Soil Microbial Characteristics,Soil Enzyme Activities and Soil Nutrient in the Winter Wheat Field[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 224-231. doi: 10.7668/hbnxb.2016.01.036.

http://www.hbnxb.net/CN/Y2016/V31/I1/224

参考文献

[1] Sinsabaugh R L,Antibus R K,Linkins A E,et al.Wood decomposition:nitrogen and phosphorus dynamics in relation to extracellular enzyme activity[J].Ecology,1993:1586-1593.
[2] Baum C,Leinweber P,Schlichting A.Effects of chemical conditions in re-wetted peats on temporal variation in microbial biomass and acid phosphatase activity within the growing season[J].Applied Soil Ecology,2003,22(2):167-174.
[3] Benitez E,Melgar R,Sainz H,et al.Enzyme activities in the rhizosphere of pepper(Capsicum annuum L.) grown with olive cake mulches[J].Soil Biology & Biochemistry,2000,32(13):1829-1835.
[4] De La Paz Jimenez M,De La Horra A,Pruzzo L,et al.Soil quality:a new index based on microbiological and biochemical parameters[J].Biology and Fertility of Soils,2002,35(4):302-306.
[5] Piotrowska A,Wilczewski E.Effects of catch crops cultivated for green manure and mineral nitrogen fertilization on soil enzyme activities and chemical properties[J].Geoderma,2012,189:72-80.
[6] Yao X H,Min H,Lü Z H,et al.Influence of acetamiprid on soil enzymatic activities and respiration[J].European Journal of Soil Biology,2006,42(2):120-126.
[7] 罗珠珠,黄高宝,Li G D,等.保护性耕作对旱作农田耕层土壤肥力及酶活性的影响[J].植物营养与肥料学报,2009,15(5):1085-1092.
[8] Bell C W,Tissue D T,Loik M E,et al.Soil microbial and nutrient responses to 7 years of seasonally altered precipitation in a Chihuahuan Desert grassland[J].Global change biology,2014,20(5):1657-1673.
[9] Adrover M,Farrús E,Moyà G,et al.Chemical properties and biological activity in soils of Mallorca following twenty years of treated wastewater irrigation[J].Journal of Environmental Management,2012,95(Suppl):S188-S192.
[10] Holt J A.Grazing pressure and soil carbon,microbial biomass and enzyme activities in semi-arid northeastern Australia[J].Applied Soil Ecology,1997,5(2):143-149.
[11] 王杰,李刚,修伟明,等.氮素和水分对贝加尔针茅草原土壤酶活性和微生物量碳氮的影响[J].农业资源与环境学报,2014,31(3):237-245.
[12] 刘善江,夏雪,陈桂梅,等.土壤酶的研究进展[J].中国农学通报,2011,27(21):1-7.
[13] Burns R G,DeForest J L,Marxsen J,et al.Soil enzymes in a changing environment:current knowledge and future directions[J].Soil Biology and Biochemistry,2013,58:216-234.
[14] Meng Q F,Sun Q P,Chen X P,et al.Alternative cropping systems for sustainable water and Nitrogen use in the North China Plain[J].Agriculture Ecosystems & Environment,2012,146(1):93-102.
[15] Liu X,Shao L,Sun H,et al.Responses of yield and water use efficiency to irrigation amount decided by pan evaporation for winter wheat[J].Agricultural Water Management,2013,129:173-180.
[16] Fang Q X,Ma L,Green T R,et al.Water resources and water use efficiency in the North China Plain:Current status and agronomic management options[J].Agricultural Water Management,2010,97(8):1102-1116.
[17] Ram H,Dadhwal V,Vashist K K,et al.Grain yield and water use efficiency of wheat (Triticum aestivum L.) in relation to irrigation levels and rice straw mulching in North West India[J].Agricultural Water Management,2013,128:92-101.
[18] Fang Q,Chen Y,Yu Q,et al.Much improved irrigation wheat-maize double use efficiency in an intensive cropping system in the North China plain[J].Journal of Integrative Plant Biology,2007,49(10):1517-1526.
[19] Guan D,Zhang Y,Al-Kaisi M M,et al.Tillage practices effect on root distribution and water use efficiency of winter wheat under rain-fed condition in the North China Plain[J].Soil and Tillage Research,2015,146:286-295.
[20] 王志敏,王璞,李绪厚,等.冬小麦节水省肥高产简化栽培理论与技术[J].中国农业科技导报,2006,8(5):38-44.
[21] 秦欣,刘克,周丽丽,等.华北地区冬小麦-夏玉米轮作节水体系周年水分利用特征[J].中国农业科学,2012,45(19):4014-4024.
[22] Bardgett R D,Lovell R D,Hobbs P J,et al.Seasonal changes in soil microbial communities along a fertility gradient of temperate grasslands[J].Soil Biology and Biochemistry,1999,31(7):1021-1030.
[23] Joergensen R G,Brookes P C.Ninhydrin-reactive Nitrogen measurements of microbial biomass in 0.5 m K₂SO₄ soil extracts[J].Soil Biology and Biochemistry,1990,22(8):1023-1027.
[24] Allison S D,Czimczik C I,Treseder K K.Microbial activity and soil respiration under Nitrogen addition in Alaskan boreal forest[J].Global Change Biology,2008,14(5):1156-1168.
[25] 王彬彬,林启美,陈源泉,等.微池板比色法在多酚氧化酶和β葡萄糖苷酶研究中的应用[J].土壤学报,2012,49(4):773-779.
[26] 关松荫.土壤酶及其研究法[M].北京:农业出版社,1986:342-344.
[27] Chen R,Senbayram M,Blagodatsky S,et al.Soil C and N availability determine the priming effect:microbial N mining and stoichiometric decomposition theories[J].Global Change Biology,2014,20(7):2356-2367.
[28] Hueso S,García C,Hernández T.Severe drought conditions modify the microbial community structure,size and activity in amended and unamended soils[J].Soil Biology and Biochemistry,2012,50:167-173.
[29] 刘振香,刘鹏,贾绪存,等.不同水肥处理对夏玉米田土壤微生物特性的影响[J].应用生态学报,2015,26(1):1.
[30] Carter M R,Rennie D A.Dynamics of soil microbial biomass N under zero and shallow tillage for spring wheat,using ¹⁵N urea[J].Plant and Soil,1984,76(1/3):157-164.
[31] 何友军,王清奎,汪思龙,等.杉木人工林土壤微生物生物量碳氮特征及其与土壤养分的关系[J].应用生态学报,2006,17(12):2292-2296.
[32] Xiang S R,Doyle A,Holden P A,et al.Drying and rewetting effects on C and N mineralization and microbial activity in surface and subsurface California grassland soils[J].Soil Biology and Biochemistry,2008,40(9):2281-2289.
[33] Sardans J,Peñuelas J,Estiarte M.Changes in soil enzymes related to C and N cycle and in soil C and N content under prolonged warming and drought in a Mediterranean shrubland[J].Applied Soil Ecology,2008,39(2):223-235.
[34] Sinsabaugh R L.Phenol oxidase,peroxidase and organic matter dynamics of soil[J].Soil Biology and Biochemistry,2010,42(3):391-404.
[35] Li F S,Yu J M,Nong M L,et al.Partial root-zone irrigation enhanced soil enzyme activities and water use of maize under different ratios of inorganic to organic Nitrogen fertilizers[J].Agricultural Water Management,2010,97(2):231-239.
[36] Brockett B F T,Prescott C E,Grayston S J.Soil moisture is the major factor influencing microbial community structure and enzyme activities across seven biogeoclimatic zones in western Canada[J].Soil Biology and Biochemistry,2012,44(1):9-20.
[37] Sardans J,Penuelas J.Soil enzyme activity in a Mediterranean forest after six years of drought[J].Soil Science Society of America Journal,2010,74(3):838-851.
[38] 刘美玉,陈益银,习向银,等.水氮耦合对烤烟生育期内土壤酶活性动态变化的影响[J].华北农学报,2011,26(5):159-163.
[39] 薛丽华,王志敏,郭志伟,等.麦田不同灌水处理对土壤酶活性时空分布的影响[J].水土保持学报,2010(5):228-232.

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