基于SNP标记技术的糯玉米种质遗传多样性分析

doi:10.7668/hbnxb.2015.03.015

摘要/Abstract

摘要： 为揭示糯玉米种质的遗传多样性,利用新一代分子标记技术-SNPs(Single nucleotide polymorphisms)对39份不同基因型的糯玉米自交系进行了基因型分析。结果表明,1 059个SNP标记在39份自交系中的多态性信息含量(PIC)为0.05~0.38,平均含量为0.31;最小等位基因频率(MAF)为0.03~0.50,平均值为0.29;期望杂合度变化范围为0.05~0.50,平均期望杂合度为0.39。39份自交系之间的亲缘关系(Kinship)系数为0.00~0.91,京6与京糯6之间亲缘关系最近。通过Neighbor-joining(NJ)聚类分析,将39份自交系划分为5大类群,其中系谱来源不清晰的糯玉米自交系均被划分至不同类群中,明确了其亲缘关系。因此,SNP标记适用于糯玉米自交系的遗传多样性分析及亲缘关系研究,可为糯玉米种质资源利用及品种选育提供参考。

关键词: 糯玉米, SNP标记, 遗传多样性, 京糯6, 白糯6

Abstract: In order to explore genetic diversity of waxy corn varieties,a novel molecular marker technology-Single nucleotide polymorphism (SNP) markers were adopted in genotypic analysis of 39 elite waxy corn inbred lines.Polymorphic information content (PIC) of 1 059 SNP markers on these inbred lines were 0.05-0.38,with average value being 0.31;Minor allele frequency (MAF) were 0.03-0.50,with average value being 0.29;Expected heterozygosity were 0.05-0.50,with an average of 0.39.Kinship coefficient between 39 inbred lines ranged from 0.00 to 0.91.Jing 6 and Jingnuo 6 had the strongest similarity.Five groups were divided from the 39 lines by Neighbor-joining (NJ) clustering method.Inbred lines with unclear pedigree were divided into different groups,so their relatedness with other lines were defined.Therefore,SNP markers were applicable to the analysis of genetic diversity and genetic relationships of waxy corn inbred lines,could provide data for utilization and developing new varieties in breeding program.

Key words: Waxy corn, SNP marker, Genetic diversity, Jingnuo 6, Bainuo 6

中图分类号:

S513.03

史亚兴, 卢柏山, 宋伟, 徐丽, 赵久然. 基于SNP标记技术的糯玉米种质遗传多样性分析[J]. 华北农学报, 2015, 30(3): 77-82. doi: 10.7668/hbnxb.2015.03.015.

SHI Ya-xing, LU Bai-shan, SONG Wei, XU Li, ZHAO Jiu-ran. Genetic Diversity Analysis of Waxy Corn Inbred Lines by Single Nucleotide Polymorphism (SNP) Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 77-82. doi: 10.7668/hbnxb.2015.03.015.

http://www.hbnxb.net/CN/Y2015/V30/I3/77

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